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Comparison of gene expression in glochidia and adult freshwater pearl mussels (Chamberlainia hainesiana) using cDNA-AFLP and differential display techniques |
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| รหัสดีโอไอ | |
| Creator | 1. Sawate Vannarattanarat 2. Utaiwan Kovitvadhi 3. Vipa Hongtrakul |
| Title | Comparison of gene expression in glochidia and adult freshwater pearl mussels (Chamberlainia hainesiana) using cDNA-AFLP and differential display techniques |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2554 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | 4 |
| Journal No. | 1 |
| Page no. | 63 |
| Keyword | freshwater pearl mussel, Chamberlainia hainesiana, differential display, cDNA-AFLP |
| ISSN | 8578664 |
| Abstract | Comparison of gene expression between glochidia and adult stages of freshwater pearl mussels, Chamberlainia hainesiana, was performed using cDNA-AFLP and differential display techniques. DNA fingerprints from cDNA-AFLP using 64 primers combination produced 16 different DNA bands between glochidia and adult samples, whereas from differential display using 136 random/oligo dT primer pairs produced 14 different DNA bands. Sequencing and BLAST analysis of all differentially expressed fragments were reported. All sequences obtained did match the sequences in mantle cDNA library of freshwater pearl mussel, Hyriopsis cumingii Lea. One DNA fragment was a part of gene coding for fasciclin-like protein, one of the genes potentially involved in pearl formation. Moreover, DNA marker specific to C. hainesiana, the high level pearl producing mussel, was developed and tested for specificity comparing to other mussels. |
