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Relationship between the 3435C>tTand 2677G>T/A polymorphysms and ABCB1 expression in peripheral blood mononuclear cells extracted from Thai volunteers |
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| รหัสดีโอไอ | |
| Creator | Patcharaporn Sudchada, Nuttawut Saelim, Sarawut Oo-puthinan, Oranun Kerdpin |
| Title | Relationship between the 3435C>tTand 2677G>T/A polymorphysms and ABCB1 expression in peripheral blood mononuclear cells extracted from Thai volunteers |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2554 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | 4 |
| Journal No. | 2 |
| Page no. | 139 |
| Keyword | ABCB1 expression, 3435C>T, 2677G>T/A, peripheral blood mononuclear cells (PBMCs) |
| ISSN | 8578664 |
| Abstract | Limited data of ABCB1 3435C>T and 2677G>T/A SNPs and its expression in peripheral mononuclear cells (PBMCs) with proper normalization of endogenous controls used is available in Thai population. The primary objective was to investigate the relationship of these SNPs and its expression in PBMCs extracted from Thai healthy individuals. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) was utilized to determine the SNPs in 30 females and 26 males. Total RNA was extracted from PBMCs followed by reverse transcription. Actin-?, ?-2M and GAPDH were internal controls. Real-time quantitative PCR was performed to determine the expression levels. We found that TT genotype of 3435C>T SNP is correlated with higher of ABCB1 expression levels with a significant covariate of gender. 3435C>T genotypes show a strong relationship to 2677G>T genotypes. Most subjects who carry TT genotype at position 3435 will also carry TT genotype at position 2677. No gender difference was noted in the distributions of these SNPs. The results show that genotypes of SNP 3435C>T and gender have, in part, influenced on the expression levels. Therefore, one who carries TT genotype of 3435C>T SNP may have lower response to drugs which target sites are PBMCs. |
