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DNA marker for A1 and A2 Musa genome identification |
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| รหัสดีโอไอ | |
| Creator | 1. Chanram Roopkham 2. Benchamas Silayoi 3. Savitr Trakulnaleamsai 4. Somsak Apisitwanich |
| Title | DNA marker for A1 and A2 Musa genome identification |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2554 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | 4 |
| Journal No. | 1 |
| Page no. | 43 |
| Keyword | banana, Musa, DNA marker, SSH |
| ISSN | 8578664 |
| Abstract | Musa acuminata Colla and Musa balbisiana Colla are expected to be ancestors of cultivated bananas which are highly variable throughout Thailand. Four genome types of Musa are reported as A, B, S and T. A new and highly effective method, suppression subtractive hybridization (SSH), has been introduced to develop markers for edible banana genome identification. Four primer pairs, SSH14, SSH21, SSH23 and SSH255, are specific to A1 genome with the size of 250, 228, 572 and 376 bp, respectively, 4 primer pairs, SSH20, SSH26, SSH66 and SSH214, are specific to A2 genome with the size of 540, 473, 393 and 534 bp, respectively. Moreover, one primer pair, SSH10, could identify the common A and B genomes with the size of 433 and 700 bp, respectively. This proved that SSH is an effective technique for identification of closely related organism. |
