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Plant Regeneration from Young Shoot of Nilegrass (Acroceras macrum) |
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| รหัสดีโอไอ | |
| Creator | 1. Anurug Poeaim 2. Sarun Sukhawat 3. Jantakarn Arananant 4. Pradit Pongtongkam |
| Title | Plant Regeneration from Young Shoot of Nilegrass (Acroceras macrum) |
| Publisher | Genetics Society of Thailand |
| Publication Year | 2552 |
| Journal Title | Thai Journal of Genetics |
| Journal Vol. | 2 |
| Journal No. | 1 |
| Page no. | 30 |
| Keyword | Nilegrass, embryogenic callus, regeneration |
| ISSN | 8578664 |
| Abstract | To optimize the method for embryogenic callus induction and plant regeneration from young shoot explantsof Nilegrass (a warm-season forage grass species), young shoots were cultured onto LS medium supplementedwith 0.5, 1, 3 and 5 mg/l 2,4-D, 0.1 mg/l BA, 4 mg/l Thiamine-HCl, 100 mg/l ?-ketoglutaric acid, 30 g/l sucrose and 2.6 g/l phytagel for 8 weeks. The highest callus formation and embryogenic callus inductionwas obtained on LS medium supplemented with 1 mg/l 2,4-D (27.50% and 13.75%, respectively). Theembryogenic nature of the compact structure was confirmed by scanning electron microscopy. Theembryogenic calli were cultured on LS media supplemented with 0.5, 1, 3 and 5 mg/l BA, 3% sucrose and2.6 g/l phytagel. After 8 weeks of culture, the highest regeneration percentages (100%) was obtained on 5mg/l BA. The plantlets were successfully transferred to grow in the soil. |
