|
Silica Gel Drying of Sea Cucumber DNA Samples is a Viable Alternative toSequencing |
|---|---|
| รหัสดีโอไอ | |
| Creator | Arom Mucharin |
| Title | Silica Gel Drying of Sea Cucumber DNA Samples is a Viable Alternative toSequencing |
| Contributor | Mats Gustafsson, Tomas Cedhagen and Peter Funch |
| Publisher | Office of Natural Science Research, National Science Museum |
| Publication Year | 2564 |
| Journal Title | The Thailand Natural History Museum Journal |
| Journal Vol. | 15 |
| Journal No. | 2 |
| Page no. | 135-136 |
| Keyword | Silica gel drying, sea cucumber, DNA, sequencing. |
| Website title | THE THAILAND NATURAL HISTORY MUSEUM JOURNAL |
| ISSN | ISSN 1686-770X |
| Abstract | The material for DNA extraction of sea cucumber which involve refrigeration may not always be convenientbecause of certain conditions that cannot be achieved. So, we tried out drying the sea cucumber body wall. Acomparison is made with traditional ethanol preservation and a modern molecular method in 3 different samplingmethods: storage in 96 % Ethanol under refrigeration; storage in RNAlater (Quiagen) under refrigeration anddrying in excess silica gel at ambient temperature. The result clearly shows that there is at least some DNA inevery samples. Even though it is not an optimial method, silica gel drying can be used for sea cucumber DNAsampling. |
