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Genome Wide Association Study (GWAS) for Southern Corn Rust (SCR) Disease Resistance in Maize |
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| รหัสดีโอไอ | |
| Creator | Nay Nay Oo |
| Title | Genome Wide Association Study (GWAS) for Southern Corn Rust (SCR) Disease Resistance in Maize |
| Contributor | Vinitchan Ruanjaichon, Kularb Laosatit, Theerayut Toojinda, Jintana Unartngam |
| Publisher | Department of Agriculture |
| Publication Year | 2567 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 42 |
| Journal No. | 1 |
| Page no. | 71-85 |
| Keyword | Genome-wide association study (GWAS), Puccinia polysora, Southern corn rust (SCR), single nucleotide polymorphosim (SNP) genotyping array |
| URL Website | tci-thaijo.org/index.php/thaiagriculturalresearch |
| Website title | Thai Agricultural Research Journal |
| ISSN | 3027-7272 |
| Abstract | Southern corn rust (SCR), caused by Puccinia polysora Undrew, is one of the most important maize diseases threatening maize production. Growing resistant varieties is the most practical and cost-effective approach to controlling the disease. Identification of resistance genes would help in the development of high-yielding resistant maize hybrids. Genome-wide association studies (GWAS) can efficiently reveal genomic loci associated with the desired phenotypic traits. In this study, the phenotypes of 262 maize recombinant inbreds against two isolates of SCR disease, namely, Nakhon Pathom and Chiang Mai, were investigated. Using 434,871 single nucleotide polymorphism (SNP) markers obtained from the maize SNP 600K genotyping array, GWAS was performed with the Fixed and random model Circulating Probability Unification (FarmCPU) model. The results showed that 19 SNPs distributed on chromosomes 1, 2, 3, 4, 5, 9 and 10 were significantly associated with resistance to SCR disease. As a result, 19 quantitative trait loci (QTL)s and 36 candidate genes were identified. In addition, the three major QTLs/SNP loci which included AX-90915192 on chromosome 4, AX-91151225 on chromosome 9 and AX-91648757 on chromosome 5, could distinguish the disease-resistant from disease-susceptible lines. These identified SNPs and genes provide useful information for cloning genes and understanding disease resistance mechanisms to SCR, and can be used in marker-assisted breeding programs to develop SCR resistant maize. |
