Metarhizium Pellet Drying Process and Efficiency in Controlling the Coconut Rhinoceros Beetle Larvae
รหัสดีโอไอ
Creator Saowanit popoonsak
Title Metarhizium Pellet Drying Process and Efficiency in Controlling the Coconut Rhinoceros Beetle Larvae
Contributor Thiphaphorn Naunnet, Pattira satwong
Publisher Department of Agriculture
Publication Year 2566
Journal Title Thai Agricultural Research Journal
Journal Vol. 41
Journal No. 2
Page no. 116-128
Keyword Metarhizium, entomopathogenic fungi, drying productio, biocontrol
URL Website https://at.doa.go.th/journal
Website title Thai Agricultural Research Journal
ISSN 0125-8389
Abstract The study of drying Metarhizium processes for fungal fresh culture pellets formulation was conducted under laboratory condition, between October 2019 to September 2021. The objective of this study was to compare two The study of drying processes for Metarhizium fungal fresh culture pellets formulation was conducted under laboratory condition, between October 2019 to September 2021. Two methods of drying; dehydrating at 50 ? 2 ?C in a hot air oven and dehydrating at 30 ? 3 ?C in a 3x3 meter sterile room were compared. The tested samples were collected after 24, 48, 72, and 96 hrs. of drying. the number of conidia, the fungal viability (coony forming unit: CFU) and the moisture content of pellets from both methods were measured. In conclusion, the best method for drying Metarhizium is the sterile room at 30 ? 3 ?C for 24 hrs. which yield highest conidia germination. Pellets obtained from this process was able to control the coconut rhinoceros beetle larvae effectively. There were no statistically significant difference in the number of conidia from both methods at 24 hrs. There were 1.09x108, 3.12x108 and 1.50x108 conidia/ml. from hot air oven method and 2.32x108, 2.55x108 and 2.18x107 conidia/ml. from sterile room method. However, the fungal viability in the sterile room drying method (6.25x107, 5.17x107 and 2.73x108 cfu/g) was higher than a those from hot air oven drying method (3.64x105, 2.65x106 and 1.95x107 cfu/g) in all three tests. The moisture content in a hot air oven drying method (1.91, 2.64 and 1.39 %) was greater than the sterile room drying method (4.77, 3.63 and 3.32 %). There was no significant difference of both methods in controlling the coconut rhinoceros beetle larvae (100 %). Considering the cost of electricity, the sterile room drying method (37.55 baht /day) costed seven times lower than ahot air oven drying method (268.10 baht/day).
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