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A Rapid, Economical and Hazardous Organic Solvent Free Method for DNA Extraction from Cassava |
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| รหัสดีโอไอ | |
| Creator | Jeeraporn Kansup |
| Title | A Rapid, Economical and Hazardous Organic Solvent Free Method for DNA Extraction from Cassava |
| Contributor | Tanavadee Kumchoo, Vipavee Chanroj, Suphawadee Ngorian, Suwaluk Amawan, Prapit Wongtiem |
| Publisher | Department of Agriculture |
| Publication Year | 2564 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 39 |
| Journal No. | 2 |
| Page no. | 189-201 |
| Keyword | DNA extraction, cassava, interfering substance, sodium dodecyl sulfate (SDS), polyvinylpyrrolidone (PVP) |
| URL Website | https://at.doa.go.th/journal |
| Website title | Thai Agricultural Research Journal |
| ISSN | 0125-8389 |
| Abstract | The extraction of good quality DNA from leaves of cassava is a complicated process due to the presence of interfering substances such as polyphenols and polysaccharides that affect the quality of DNA. In hexadecyltrimethylammonium bromide or CTAB method which is widelyusedfor DNA extraction,hazardousorganic solvents suchasphenol andchloroform are required to remove these interfering substances. The whole process does take approximately 130 minutes making it unsuitable for extracting large number of DNA samples. The objective of this research was to develop a DNA extraction method from cassava leaves which is rapid, free of harmful organic solvents, does not use liquid nitrogen, as well as cost and time effective. The DNA extraction method developed in this research was based on sodium dodecyl sulfate/sodium chloride or SDS/NaCl method. The SDS/NaCl+PVP method yielded DNA amount in the range of 1,431.4 - 1,510.3 ตg per 1g of leaf sample with absorption ratios A260/A280 of 1.74 - 1.90. Agarose gel electrophoresis showed the intact, sharp and clear |
