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Marker-Assisted Selection for Resistance to Cassava Mosaic Disease in Manihot esculenta Crantz |
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| รหัสดีโอไอ | |
| Creator | Jeeraporn Kansup |
| Title | Marker-Assisted Selection for Resistance to Cassava Mosaic Disease in Manihot esculenta Crantz |
| Contributor | Suwaluk Amawan, Prapit Wongtiem, Aroonothai Sawwa, Suphawadee Ngorian, Danai Narkprasert, Jinnajar Hansethasuk |
| Publisher | Department of Agriculture |
| Publication Year | 2563 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 38 |
| Journal No. | 1 |
| Page no. | 68-79 |
| Keyword | Cassava (Manihot esculentaCrantz), Cassava mosaic disease (CMD), Marker-assisted selection |
| URL Website | http://at.doa.go.th/journal |
| Website title | Thai Agricultural Research Journal |
| ISSN | 0125-8389 |
| Abstract | Cassava Mosaic Disease (CMD) is a very important disease that causes severe damage to cassava production. The objective of this research was to select cassava varieties resistant to CMD by using molecular markers.Two hundred and fifty varieties fromRayong Field Crops Research Center were collected and examined using molecular markersin the laboratory of Biotechnology Research and Development Office, Department ofAgriculture from October 2017 to September 2019. In this research, three groups ofmolecular markers were used in selection for resistance to CMD. Group 1 included 4Sequence Characterized Amplified Region (SCAR) and Simple Sequence Repeat markers (SSR),namely RME1, NS158, SSRY28 and NS169 which are flanking nearby the CMD resistancelocus called CMD2. Group 2 consisted of 2 molecular markers, namely EST-LRR andNB-ARC domains-containing disease resistance protein (EST-R protein) and EST-Proteinkinase superfamily protein (EST-Kinase) which were obtained from the expressed sequencetags of genes in response to cassava mosaic virus, the cause of CMD. Group 3 contained3 positions of Single Nucleotide Polymorphism (SNP) markers in Peroxidase gene, namelyEx2-78, Ex2-157 and Ex3-128. In total, 9 molecular markers were used to search forcassava varieties showing DNA band patterns and nucleotide sequences similar to those ofthe CMD resistant variety named TME3. Results of the experiment revealed that there were2 varieties that showed the same DNA band patterns and nucleotide sequences as TME3in all 9 molecular markers, namely MMAL63 and CMR23-149-59. These varieties may beresistant to CMD. However, it is necessary to test their resistance against the actualcassava mosaic virus before using them in the variety development scheme, and propagatefor farmers. |
