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Detection of Chrysanthemum stunt viroid (CSVd) from Chrysanthemum Plants in the Fields |
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| รหัสดีโอไอ | |
| Creator | Chamaporn Netwong |
| Title | Detection of Chrysanthemum stunt viroid (CSVd) from Chrysanthemum Plants in the Fields |
| Contributor | Kanokmanee Tansuwan, Kanungnit Reanwarakron |
| Publisher | Department of Agriculture |
| Publication Year | 2563 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 38 |
| Journal No. | 1 |
| Page no. | 23-32 |
| Keyword | Viroid, chrysanthemum, RT-PCR, Sequencing |
| URL Website | http://at.doa.go.th/journal |
| Website title | Thai Agricultural Research Journal |
| ISSN | 0125-8389 |
| Abstract | Twenty-three chrysanthemum samples showing stunting, chlorotic leaves, small andabnormal flowers were collected from the field grown plants in Wang Nam Khiao district, Nakhon atchasima province and determined for the presence of Chrysanthemum stuntviroid (CSVd) by using reverse transcription polymerase chain reaction (RT-PCR) technique.Eight samples produced DNA products of about 360 basepairs. Cloning and sequencing of these DNA fragments revealed 354 nucleotides in size. All of them were 100% identical, and were identified as Chrysanthemum stunt viroid based on criteria of 98-100% identity to CSVd accessions previously recorded in GenBank as AF394452, JQ685739, HQ891018, JX909290, JF414238, JF398538, Z68210, DQ094298, FN646407 and U82445. For bioassay,tomato cv. Rutgers and chrysanthemum plants were inoculated with sap from CSVd-infected plant. The Rutgers tomato were symptomless while chrysanthemum plants showed chlorosis, small leaves and leaf distortion. All these bioassayed plants were confirmed for CSV dinfection by RT-PCR. This is the first report of CSVd natural infection of chrysanthemum in Thailand. |
