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Assessment of Resistance to Squash leaf curl Yunnan virus in Some Cucurbitaceous Plants |
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| รหัสดีโอไอ | |
| Creator | 1. Nattaporn Butnut1 2. Wichai Kositratana 3. Sujin Patarapuwadol |
| Title | Assessment of Resistance to Squash leaf curl Yunnan virus in Some Cucurbitaceous Plants |
| Publisher | Department of Agriculture |
| Publication Year | 2562 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 37 |
| Journal No. | 1 |
| Page no. | 14-26 |
| Keyword | ฟักทอง, ฟักแฟง, บวบเหลี่ยม, Squash leaf curl Yunnan virus, พืชต้านทานโรค, Pumpkin, Wax gourd, Ridge gourd, Squash leaf curl Yunnan virus, Disease Resistant |
| URL Website | http://at.doa.go.th/journal |
| Website title | Thai Agricultural Research Journal |
| ISSN | 0125-8389 |
| Abstract | The distinct species of begomovirus named Squash leaf curl Yunnan virus (SLCuYV) was detected in Thailand. It caused yellow leaf curl diseases of commercial varieties of various cucurbitaceous plants and led to severe losses in both fruit quality and yield. The virus spreads widely across the country due to the effective transmission by whiteflies (Bemisia tabaci). Breeding for resistance of plant is the most effective approach to reduce disease loss. The aim of this study was to assess the resistance of pumpkin, wax gourd and ridge gourd cultivars to infection by SLCuYV isolateTH-PK-2018 (SLCuYV-TH-PK-2018) under greenhouse conditions. A total of 152 accessions including 106 of pumpkin (Cucurbita moschata), 39 of wax gourd (Benincasa hispida), and 7 of ridge gourd (Luffa acutangula) were evaluated for SLCuYV infection by whitefly transmission. Ten to fifteen adult whitefies were provided a 48 hour acquisition access period (AAP) initially on the symptomatic pumpkin plants, and then transferred to each healthy seedling at the first true leaf stage for a 72 hour inoculation access period (IAP). The presence of SLCuYV in inoculated plants was determined by using the enzyme-linked immunosorbent assay (ELISA) and the symptoms were scored at 4 weeks post inoculation. Virus infection index based on percentage of disease index and vulnerability index classified 20 resistant accessions including 3 of pumpkin, 10 of wax gourd and 7 of ridge gourd. |
