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Characterizations of Colletotrichum spp., Pathogens on Mango Fruits (Mangifera indica L. cv. 'Nam Dok Mai') |
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| รหัสดีโอไอ | |
| Creator | 1. Sasivimol Laksanaphisut 2. Pattavipha Songkumarn 3. Somsiri Sangchote |
| Title | Characterizations of Colletotrichum spp., Pathogens on Mango Fruits (Mangifera indica L. cv. 'Nam Dok Mai') |
| Publisher | Department of Agriculture |
| Publication Year | 2562 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 37 |
| Journal No. | 2 |
| Page no. | 197-215 |
| Keyword | Colletotrichum gloeosporioide, C. acutatum, anthracnose, mango |
| URL Website | http://at.doa.go.th/journal |
| Website title | Thai Agricultural Research Journal |
| ISSN | 0125-8389 |
| Abstract | Mango anthracnose disease is caused by several species of Colletotrichumincluding C. gloeosporioides, C. acutatum, C. siamense and C. asianumand. The disease isconsidered as one of the major constraints of Thai mango export. However, the classificationof these pathogens was still unclear. Generally, Colletotrichum spp. are classified based ontheir morphology. The aims of this study were to characterize and confirm the species ofColletotrichum isolates collected from mango orchards for exporting located in 6 differentprovinces of Thailand. The characterization was based on pathogenicity test, morphologicaland molecular characteristics. Forty four isolates were obtained from the symptomatic fruits.Pathogenicity test of all isolates showed typical anthracnose symptoms on the testedmango fruits and Koch's postulates were fulfilled by re-isolation from the inoculated fruits.The morphological characterization identified all isolates as C. gloeosporioides with6 morphotypes, as their conidia were hyaline, cylindrical with rounded-end and approximately14.90x4.02 ?m in size. Setae on infected tissue were also observed is some isolates. Speciesspecific primer analysis could confirm 39 isolates of Colletotrichum as C. gloeosporioides.Ribosomal DNA (rDNA) gene sequencing and homology analysis of other five isolates revealedthat these isolates were C. gloeosporioides except for the WH9 isolate which was identifiedas C. acutatum. By combining the results from molecular analysis with morphologicalcharacterization and pathogenicity test, we report that C. gloeosporioidesis is the maincausal pathogen of mango anthracnose in the mango orchards for exporting in Thailand. |
