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NHX1 Gene in Sugarcane cv. KPS94-13 and Wild Sugarcane and Gene Expression Pattern in Mimicking Saline Soil |
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| รหัสดีโอไอ | |
| Creator | 1. Tanawan Promkhlibnil 2. Chanakan Laksana 3. Sontichai Chanprame |
| Title | NHX1 Gene in Sugarcane cv. KPS94-13 and Wild Sugarcane and Gene Expression Pattern in Mimicking Saline Soil |
| Publisher | Department of Agriculture |
| Publication Year | 2562 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 37 |
| Journal No. | 2 |
| Page no. | 165-176 |
| Keyword | การบ่งชี้ยีน, การแสดงออกของยีน, แอนติโพทเตอร์, โซเดียมคลอไรด์ อ้อย, gene identification, gene expression, antiporter, sodium chloride, sugarcane |
| URL Website | http://at.doa.go.th/journal |
| Website title | Thai Agricultural Research Journal |
| ISSN | 0125-8389 |
| Abstract | Saline soil affects both plant growth and productivity especially sugarcane which isan economic crop but sensitive to saline soil. One of sugarcane breeding program's goalsis to improve salt tolerance in sugarcane cultivars. The objectives of this research were toclone and identify the NHX1 gene from a commercial sugarcane cv. KPS94-13 and wildsugarcane, and to study the expression pattern of the genes in salt stress condition. It wasfound that the full length of the gene was 1,623 bp. The comparison of the sequence tonucleotide data base of GenBank found that the sequence was similar to that of NHX1 geneof many monocotlydon plants. We named the sequence cloned from KPS 94-13 andwild sugarcane as SoNHX1KPS and SsNHX1WT, respectively. The sequence encoded apolypeptide of 540 amino acid residues. There were 5 amino acid residues differencebetween the amino acid sequences deduced from the genes of the two sugarcane species.The phylogenetic tree analysis indicated that the SoNHX1KPS and SsNHX1WT wereclosely related to AtNHX1. The analysis of hydrophobicity found 11 transmembrane domainsin both polypeptide sequences. Gene expression study of NHX1 in the two kinds ofsugarcane used in this experiment revealed that when subjected to 100 mM and 200 mM ofNaCl for 5 days the expression patterns of the NHX1 gene depended on the concentrationsof NaCl. The expression levels of the gene in roots and leaves of wild sugarcane werehigher than those of KPS94-13 and the expression levels of the gene in leaves were higherthan those in roots. |
