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Effect of Light to Expression Control of Eucalyptus KORRIGAN Promoter in Tobacco |
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| รหัสดีโอไอ | |
| Creator | 1. Artit Vimoltust 2. Wichai Kositratana 3. Srimek Chowpongpang |
| Title | Effect of Light to Expression Control of Eucalyptus KORRIGAN Promoter in Tobacco |
| Publisher | Department of Agriculture |
| Publication Year | 2562 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 37 |
| Journal No. | 2 |
| Page no. | 128-138 |
| Keyword | KORRIGAN โปรโมเตอร์, การแสดงออกของยีน, แสงและความมืดในการควบคุมยีน กระบวนการสังเคราะห์เซลลูโลส, KORRIGAN Promoter, Gene expression, Light and dark gene control Cellulose Biosynthesis |
| URL Website | http://at.doa.go.th/journal |
| Website title | Thai Agricultural Research Journal |
| ISSN | 0125-8389 |
| Abstract | Cellulose is the most important structural component of plant cell wall. Due to theenormous economic benefit of tree cellulose for paper, pulp, lumber and packaging industries,understanding the mechanism of cellulose biosynthesis is important for the sustainability ofthese industrial businesses. KORRIGAN gene (Kor) encodes a plasma membrane boundenzyme, endo1, 4- -D-glucanase, which is member of cellulose biosynthesis genes. Thisresearch focused on the analysis of the promoter region of the Kor gene, promoter structureand gene expression control under dark and light conditions. The Kor promoter region ofEucalyptus grandis, E. camaldulensis, and E. urophylla were cloned and sequenced.Sequences were analyzed by alignment against the E. glandis genome sequences using aClustalW 2.1 program. The gene expression control of Kor promoter was tested, recombinantplant transformation plasmid, pCAMBIA1304, with the Kor promoter fused to the reportergene, -glucuronidase (gusA) was transferred to tobacco (Nicotiana tabacum) and the geneexpression controls were analyzed by real time PCR. Results revealed that Kor promoternucleotide sequences from E. grandis, E. camaldulensis, and E. urophylla, are high similar toE. grandis Kor promoter database at the percentages of 97, 95 and 96 respectively. Twotransgenic tobacco lines, 15k2 and 15K3, showed the specific expressions of the reportergene, gusA, but limited only in the vascular tissues, and the levels of gene expression wereregulated by light, Under lighting conditions of 8 and 16 hrs. expression reduction from thoseunder dark condition of 29%, 36%, 36% and 31% respectively were observed. |
