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Identification of Protein QTLs for High Protein Soybean Breeding Using SSR Markers |
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| รหัสดีโอไอ | |
| Creator | 1. Jeeraporn Kansup 2. Pongsakorn Sunvittayakul 3. Areerat Prapech 4. Khanitha Wongwathrat 5. Supanun Janpraob 6. Chayanit Distabanjong 7. Danai Nakprasert 8. Chitima Yathaputhanon 9. Somsak Srisomboon 10. Kingkarn Ptichayakul |
| Title | Identification of Protein QTLs for High Protein Soybean Breeding Using SSR Markers |
| Publisher | Department of Agriculture |
| Publication Year | 2561 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 36 |
| Journal No. | 1 |
| Page no. | 2-15 |
| Keyword | soybean (Glycine max (L.) Merr.), seed protein content, quantitative trait loci (QTL), simple sequence repeat (SSR) markers |
| URL Website | http://at.doa.go.th/journal |
| Website title | Thai Agricultural Research Journal |
| ISSN | 0125-8389 |
| Abstract | The objective of this study was to identify quantitative trait loci (QTL) governingsoybean protein content using simple sequence repeat (SSR) markers which linked to theprotein?s character. The SSR markers were used in soybean breeding to select high proteinvarieties rapidly and accurately. A population of 4 recombinant inbred lines (RILs) derivingfrom crosses between Thai soybean varieties with different seed protein contents (C5-2,C42-3 as female parents and S1-3, S17-3 as male parents) was used to QTL analyzes. DNAfrom the parents was screened with 218 SSR markers and 106 markers showedpolymorphisms in the parents. The polymorphic markers were used in genotyping to findout cross-pollinated F1 plants. The segregation in F2 generation was confirmed byobservation of pubescence color with 3:1 ratio which brown pubescence was controlledby a single dominant gene. To generate F3 ? F7 of RILs, single seed descent method wasused. One hundred and six markers, which showed polymorphisms in the parents, wereused to identify QTL associated with soybean protein content. PCR results from F7 RILpopulation and percent protein content in F8 seeds were assembled to use in QTLs analysis.Four QTLs for protein content were localizedSatt184, Satt590, Satt196 and Satt247.The information related to these markerswill be very useful for breeding process ofhigh protein soybean and also to findnovel candidate genes which areimportant to protein content in soybeanseed of Thai soybean varieties. |
