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Identification and DREB1 Gene Expression Analysis in Response to Early Water Deficits in a Drought Tolerance Hybrid Maize Nakhon Sawan 3 |
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| รหัสดีโอไอ | |
| Creator | 1. Payungsak Rauyaree 2. Suriphat Thaitad |
| Title | Identification and DREB1 Gene Expression Analysis in Response to Early Water Deficits in a Drought Tolerance Hybrid Maize Nakhon Sawan 3 |
| Publisher | Department of Agriculture |
| Publication Year | 2561 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 36 |
| Journal No. | 1 |
| Page no. | 81-94 |
| Keyword | Maize variety Hybrid Nakhon Sawan 3, DREB1, drought tolerant genes, Quantitative Real-time Polymerase Chain Reaction, ข้าวโพดเลี้ยงสัตว์ลูกผสมพันธุ์นครสวรรค์ 3 |
| URL Website | http://at.doa.go.th/journal |
| Website title | Thai Agricultural Research Journal |
| ISSN | 0125-8389 |
| Abstract | DREB1 (Dehydration-responsive element binding protein 1) gene expressionanalysis of drought tolerance Hybrid Maize Nakhon Sawan 3 cultivar under waterdepletion had been identified and analyzed by Annealing Control Primers (ACP)-basedpolymerase chain reaction and by Quantitative Real-Time polymerase chain reactionmethods. The experiments were conducted at the Biotechnology Research andDevelopment Office, Department of Agriculture during October 2011 to September 2014.To study the DREB1 gene expressed during drought stress, DREB1 gene sequence encoding661 bp of transcription factor DREB1 and gene sequence encoding 519 bp of transcriptionfactor AP2-EREBP DREB1 were isolated. Both sequences were deposited in the Genbankdatabase with accession number KY678233 and KY678234, respectively. In addition,comparative gene expression analysis of DREB1 gene in comparison to ABRE (ABAresponsiveelement) binding protein and Elongation Factors were analyzed. Furthermore,DREB1 gene expression profile of drought tolerance Nakhon Sawan 3 cultivar and thecontrol plant at 24, 48, 72 and 96 hr under water depletions from three weeks old ofmaize leaves following early water deficits were analyzed in this study. Considering theQuantitative Real-Time-PCR analysis of DREB1, ABRE and eEF (the housekeeping gene), theresults showed that the gene expression level of DREB1 was higher than that of ABRE andeEF genes. This result suggested that transcription factors DREB1 genes were expressedduring water depletions in drought tolerance hybrid Maize Nakhon Sawan 3. Based onthese findings, this information may lead to the further study in molecular markers, andcandidate genes for drought tolerance traits in plants in the future. |
