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Molecular Markers for Analysis of Genetics Diversity and Identification of Oil Palm Hybrid Varieties |
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| รหัสดีโอไอ | |
| Creator | 1. Hathairat Urairong 2. Ornrat Wongsri 3. Naiyanate Jaroensanit Tanaka |
| Title | Molecular Markers for Analysis of Genetics Diversity and Identification of Oil Palm Hybrid Varieties |
| Contributor | Department of Agriculture |
| Publisher | Department of Agriculture |
| Publication Year | 2560 |
| Journal Title | THAI AGRICULTURAL RESEARCH JOURNAL |
| Journal Vol. | 35 |
| Journal No. | 2 |
| Page no. | 117-135 |
| Keyword | molecular markers, genetics diversity, oil palm hybrid Tenera |
| URL Website | http://at.doa.go.th/journal |
| Website title | THAI AGRICULTURAL RESEARCH JOURNAL |
| ISSN | 1258389 |
| Abstract | The genetic diversity studies and SHELL type analysis of oil palm are achieved bymolecular marker. Thirteen simple sequence repeat (SSR) markers loci were selected todistinct 10 oil palm populations viz. Deli Dura, AVROS, Yangambi, Nigeria, Calabar, Ghana,Ekona, DAMI, Tanzania and La Me. The results showed that male parent oil palm, La Mehad the most genetical differentiation from other male and female parent populations,followed by Calabar, Nigeria, Tanzania and Ghana. Among male parent oil palms, AVROShad the most genetic similarity to female parent, Deli Dura followed by DAMI. In addition,the new SSR markers were reselected for investigating the genetic diversity of Deli Durapopulations as well as the progenies derived from inter-specific hybridization betweenElaeis guineensis and E. oleifera. The obtained data are efficient to differentiate all oil palmpopulations. Moreover, the primers mEgCIR3428, mEgCIR3519 and mEgCIR0874, weresufficient to be used as markers in identifying Surat Thani 1-8 oil palm varieties. Foranalysis of oil palm SHELL type, the MADS-box gene of 129 samples including 3 shelltypes, Dura, Pisifera and Tenera from 10 distinct oil palm populations were sequencedand performed multiple nucleotides alignment. The SNPs that can differentiate the oilpalm SHELL type in each population were discovered as follow, SNPENGC (T/C) in EkonaGhana Calabar and Nigeria, SNPTaYa (A/T) in Tanzania Yangambi, SNPDA (C/G) in DAMI, SNPLaAv(C/A) in La Me and AVROS, SNPTan (C/G) in Tanzania. The obtained data of SNPs loci wereused to generate 4 sets of primers and probes for accurately and rapidly determining oil palm SHELL type with real-time PCR. These markers are very applicable for quality controlof Tenera oil palm seedling production for reducing or eliminating Dura contamination, and distinguishing the female and male parent genotype efficiently. |
