Improvement of New Hybrid Pleurotus sp. Bhutan Strains Using Di-mon Mating Technique
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Creator 1. Ratchadaporn Thonghem
2. Suvalux Chaichuchote
3. Tharinee sukomol
4. Upathum Oonjai
5. Thanchanok Natai
6. Srirat Bupato
7. Phattaya Thepdet
Title Improvement of New Hybrid Pleurotus sp. Bhutan Strains Using Di-mon Mating Technique
Contributor Department of Agriculture
Publisher Department of Agriculture
Publication Year 2560
Journal Title THAI AGRICULTURAL RESEARCH JOURNAL
Journal Vol. 35
Journal No. 3
Page no. 257-269
Keyword Bhutan Mushroom strain, hybrid strain, monokaryotic mycelium, dikaryotic mycelium, Di-mon mating
URL Website http://at.doa.go.th/journal
Website title THAI AGRICULTURAL RESEARCH JOURNAL
ISSN 1258389
Abstract Improvement of Pleurotus sp. mushroom (Bhutan strain) was done by Di-monmating technique using dikaryotic mycelium from strain No. 3 (P3) and monokaryoticmycelium from 8 isolates (A15 - A22) Eighteen hybrid strains were obtained. Aftercultivating in 800 g of sawdust plastic bags at mushroom house (Bangkok) duringNovember 2014 - February 2015, all hybrids produced the fruiting bodies. Whereas,cultivation between March and June 2015, 8 hybrid strains formed the fruiting bodies andonly 15 hybrid strains produced the fruiting bodies when cultivated during July October2015. Three hybrids, P3xSE5, P3xSA6 and P3xSB24 gave higher yield than others and wereselected for further studies. The mentioned three hybrids were cultivated in the 3 areasof Royal Project. Yield of those hybrids were compared to yield of P3 strain. Resultsrevealed that P3xSB24 was suitable for cultivation in the Royal-initiated Phu SingAgricultural Development Centre, Srisaket Province, with yield 216.82 g/bag. The 3 hybridsstrains were suitable for cultivation in Pluak Daeng, Rayong Province Development ServiceCenter Under His Majesty's Initiatives. Yields of the 3 hybrid strains were significantlyhigher than P3 in two cultivation periods. Cultivation in Kung Krabaen Bay RoyalDevelopment Study Center, Chanthaburi Province, P3xSE5 produced the highest yieldduring December 2015 March 2016, which was 171.43 g/bag.
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