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Fruit Rot Disease of Rambutan and Its Control by Bacterial Antagonist |
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| รหัสดีโอไอ | |
| Creator | 1. Boonyawadee 2. Chirawut Amara 3. Chinaphuti 4. Ratta Suttayakom |
| Title | Fruit Rot Disease of Rambutan and Its Control by Bacterial Antagonist |
| Contributor | Department of Agriculture |
| Publisher | Department of Agriculture |
| Publication Year | 2560 |
| Journal Title | THAI AGRICULTURAL RESEARCH JOURNAL |
| Journal Vol. | 35 |
| Journal No. | 3 |
| Page no. | 229-242 |
| Keyword | antagonist, Bacillus, fruit rot disease, rambutan |
| URL Website | http://at.doa.go.th/journal |
| Website title | THAI AGRICULTURAL RESEARCH JOURNAL |
| ISSN | 1258389 |
| Abstract | Fruit rot disease caused by Lasiodiplodia theobromae, Gliocephalotrichum spp.,Colletotrichum gloeosporioides, Pestalotiopsis sp. and Phomopsis sp. is the major problem toreduce quality and shelf life of harvested rambutan fruits. This research was conducted todetermine the effectiveness of bacterial antagonists to control fruit rot disease in rambutanfruit. Ten isolates of bacterial antagonists were isolated from peanut, dried longan, crown ofbanana and red kidney bean. The pre-inoculated treatment was conducted by spraying 10isolates of bacterial antagonists with concentration at 108 cfu/ml on L. theobromaeinoculated rambutan fruits for 18 h which compared to water or imazalil at 500 mg/l spraying(as control). The results showed that isolates of DL9, PN10 and DL7 could inhibit diseaseseverity by 57.82, 55.82 and 52.97 % respectively, whilst imazalil at 500 mg/l inhibited thedisease severity only 16.91% and 0% for water treatment. Then the isolates of PN10, DL7 andDL9 were identified as Bacillus spp. which were gram-positive rods, endospore-forming andcatalase positive. API test kit and molecular analysis were used to identify these 3 isolates.The 16S rDNA gene sequence of DL7 strain was 100 % similar to Bacillus siamensis and PN10and DL9 were closely related to B. siamensis and B. amyloliquefaciens sub sp. plantarum(99.91 and 99.86 % identity, respectively). After that crude extract were tested for cytotoxicityagainst Vero cell (African green monkey kidney), cytotoxic was not found. Subsequently, eachbacterial antagonist strains were individually developed into 3- different powder formulations.After 6 months storage at room temperature, the result showed that the formulation of 100 grice flour mixed with 1 ml soybean oil and 10 g sucrose was the best carrier to maintainbacterial survival. The survivals of PN10, DL7 and DL9 were 7.55 x 108, 3.73 x 108 and 8.53 x107 cfu/g respectively. The efficacy of this bio- agent formulation to control rambutan fruit rotdisease was not significantly different from using antagonist directly. |
