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In vitro Tetraploid Induction of Chrysopogon zizanioides (L.) Roberty to Improve Salt Tolerance |
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| รหัสดีโอไอ | |
| Creator | 1. Mattanaporn Maikami 2. Lily Kaveeta 3. Surin Peyachoknagul 4. Malee Nanakorn |
| Title | In vitro Tetraploid Induction of Chrysopogon zizanioides (L.) Roberty to Improve Salt Tolerance |
| Publisher | Department of Agriculture |
| Publication Year | 2560 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 35 |
| Journal No. | 1 |
| Page no. | 31-48 |
| Keyword | Vetiver grass, tetraploid, colchicine, salt tolerance |
| URL Website | http://at.doa.go.th/journal |
| Website title | Thai Agricultural Research Journal |
| ISSN | 0125-8389 |
| Abstract | Vetiver grass is being used worldwide for soil and water conservation, however,there are no report of recommended salt tolerant germplasms in Thailand. Polyploidinduction by colchicine treatment is an efficient technique used to improve abiotictolerance of many plant species. Therefore, tetraploid of vetiver was induced via invitro shoots according to a factorial in completely randomized design with two factors(four colchicine concentrations; 0, 0.1, 0.2 and 0.3% and four treatment durations; 12,24, 36 and 48 h) and the DNA content was verified by flow cytometry. It was foundthat the highest tetraploid induction efficiency was 22.22% at the treatment of 0.3%colchicine for 12 h, providing 93.8% survival rate. Total of 53 tetraploid out of 607survived shoots were further studied for stomatal characteristics and determined fortheir salt tolerance. The significant difference of guard cell length between tetraploidand diploid indicated that it can be used as a primary criterion for screening tetraploidvetiver from a large population of colchicine-treated shoots. Salt tolerance examined invitro at 0, 1.75, 2.00, 2.25 and 2.50% NaCl (w/v) revealed that survival rate of mosttetraploid accessions was higher than that of the original diploid. To confirm their salttolerance, four highest salt tolerant tetraploid accessions and the original diploid weretransplanted to a natural saline area in Dan Khun Thod District, Nakhon RatchasimaProvince for 12 months. The survival rate of selected tetraploid accessions was higherthan that of the original diploid and two selected accessions were able to survivethroughout the experimental period. This result confirmed that tetraploid induction bycolchicine treatment was successfully enhance salt tolerance of vetiver grass. |
