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Efficiency of Bioproduct from Bacillus subtilis Isolate 20W1 to Control Alternaria brassicicola a Causal Agent of Chinese Kale Leaf Spot |
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| รหัสดีโอไอ | |
| Creator | 1. Boossaracum Udomsak 2. Nuttima Kositcharoenkul 3. Sureeporn Bua-art 4. Buranee Puawongphat 5. Rossukon Rungjang |
| Title | Efficiency of Bioproduct from Bacillus subtilis Isolate 20W1 to Control Alternaria brassicicola a Causal Agent of Chinese Kale Leaf Spot |
| Publisher | Department of Agriculture |
| Publication Year | 2560 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 35 |
| Journal No. | 1 |
| Page no. | 2-13 |
| Keyword | โรคใบจุดคะน้า, จุลินทรีย์ปฏิปักษ์, ชีวภัณฑ์, Bioproduct, Bacillus subtilis, Alternaria brassicicola, Chinese Kale, Leaf Spot |
| URL Website | http://at.doa.go.th/journal |
| Website title | Thai Agricultural Research Journal |
| ISSN | 0125-8389 |
| Abstract | Alternaria brassicicola can cause leaf spot symptoms at every growth stages ofChinese kale. Biological control is an alternative technique to reduce chemical usage.However, a limiting factor for biological control is the un-survival of antagonists onplant leaf surface. Bacillus bacteria can be a promising antagonist to A. brassicicola,which has a special ability in producing endospores that could tolerance and grewwell in field condition. During October 2011 ? September 2013, 135 isolates of Bacillusspp. isolated from soil, manure, and planting materials, were evaluated for the ability toinhibit growth of A. brassicicola using dual culture technique. Five isolates effectivelyinhibited mycelia growth of A. brassicicola, namely 20W1 20W5 20W4 20W12 and17G18. These isolates were then tested for the disease control in the screen house byspraying of its cell suspension prior to the inoculation of the pathogen. We found thatall isolates could effectively reduce Chinese kale leaf spot disease to 46.77%, 52.81%,59.99%, 60.45% and 71.31% respectively compared to 73.79% of the disease in nonantagonistspraying treatment. Field trial at Ta Maka District, Kanchanaburi provincealso showed results as similar as in screening house experiment. Those 5 isolates ofBacillus spp. were formulated into powder formulation and tested again at the samefield. The results showed that all isolatescould significantly control the diseasebetter than a non-antagonist sprayingtreatment. Isolate of B. subtilis 20W1was the most effective antagonist whichreduced the disease to 32.88%. Efficacytrial of the 20W1 isolate at 20-30 g/20 Lof water showed the disease control atthe same level as using mancozeb 80%WP at 40 g/20 L of water and significantlycontrolled the disease when applied at40-50 g/20 L of water. In conclusion,B. subtilis 20W1 is a new potentialisolate which can be developed to thebio-fungicide for a commercial scale. |
