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Development on Saponin Extraction from Rambutan Peel and the Efficacy Test of Saponln |
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| รหัสดีโอไอ | |
| Creator | 1. Apiradee Korpphaiboon 2. Orwintinee Chusri 3. Siriporn Tengrang |
| Title | Development on Saponin Extraction from Rambutan Peel and the Efficacy Test of Saponln |
| Publisher | Department of Agriculture |
| Publication Year | 2560 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 35 |
| Journal No. | 1 |
| Page no. | 60-73 |
| Keyword | วิธีการสกัดหยาบ, สารสกัดหยาบซาโปนิน, ควบคุมศัตรูพืช, หอยเชอรี่, เชื้อราโรคพืช, ?????????????, crude extraction methods, crude saponin, pest control, Pomacea canaliculata, fungal plant pathogens |
| URL Website | http://at.doa.go.th/journal |
| Website title | Thai Agricultural Research Journal |
| ISSN | 0125-8389 |
| Abstract | Development on saponin extraction from rambutan peel and its efficacy testwas carried out aiming to gain the most effective technique for this. Hence, agriculturalwaste as such rambutan peel can be utilized. The research was conducted atChanthaburi Horticultural Research Center during September 2011 to October 2014. Itwas included testing and verifying of extraction techniques, identification andquantification of saponin, and efficacy test of saponin. Two extraction techniques,soaked and reflux extraction, using 3 solvents, 70% ethanol, 70% methanol anddistilled water, were compared. The crude extract of which rambutan peel obtainedfrom reflux technique compared with soaked extraction technique for nine daysshowed that higher the extract dry weight 44% and 33% for only nine hours.Theextracts were identified as triterpene and steroid saponin by FTIR. Spectrophotometerwas used to quantify the amount of saponin which the reflux extraction using 70%methanol yielded the highest saponin (422.05 mg/g). Therefore, it is the most effectiveextraction technique as it yielded more amount of saponin than distilled water and70% ethanol reflux methods, 23% and 14% respectively. Efficacy test indicated thatapplied saponin solution at 2,000 and 4,000 mg/L to channeled apple snail (Pomaceacanaliculata) could effectively kill the snail within 12 h. Moreover, when mixed saponinwith PDA at 2,000 mg/L, it could effectively inhibited growth of the 3 fungal pathogen,Phytophthora palmivora, Colletotrichum spp. and Marasmius palmivorus Sharples,compared to control. |
