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Identifif ication of Embellisia allii Causing Skin Blotch on Garlic Bulbs Imported from The People's Republic of China and Controlling by Ozonated water |
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| รหัสดีโอไอ | |
| Creator | 1. ThurdpanTummarattanapong 2. SiamPopluechai Saranyaphat 3. BoonmeeKevin D.Hyde |
| Title | Identifif ication of Embellisia allii Causing Skin Blotch on Garlic Bulbs Imported from The People's Republic of China and Controlling by Ozonated water |
| Publisher | Department of Agriculture |
| Publication Year | 2559 |
| Journal Title | THAI AGRICULTURAL RESEARCH JOURNAL |
| Journal Vol. | 34 |
| Journal No. | 3 |
| Page no. | 230 |
| Keyword | โรคเปื้อนดำ, กระเทียม, น้ำโอโซน, Embellisia allii, Skinblotch, Garlic, ozone |
| ISSN | 1258389 |
| Abstract | ThestudyofskinblotchpathogenofgarlicbulbimportedfromThePeople'sRepublicofChinawascarriedoutatChiangsaenPlantQuarantineStation,ChiangRaiProvinceduringJanuary-August2016.Symptomofskinblotchwasdetectedin15of187samplesofimportedgarlicbulbs.Embellisia allii wasisolatedasakeypathogencausedskinblotchsymptominall15defectedsamplesusingmorphologicalandmolecularcharacteristics.ThemolecularcharacteristicwasinvestigatedbyaPCRandrunwithgelelectrophoresis.TheresultsshowedthatfungalDNAof15isolateswereconsistedof315basepairs.ObtainedbasesequencewascomparedtotheGenbankdatabase.ItwasfoundthatskinblotchfungifromgarlicbasesharedthesameE. alliifor100%identity.The E. allii isquarantinepestinThailand,thereforecontrolofE. alliimustbeconcerned.Applicationofozonatedwaterat1g/hfor30,45and60mincouldreducethesporesgerminationofE. alliiafter24hofincubationat0.75,0and0%respectively.Moreover,applicationofozonatedwaterat1g/hfor30minwascompletelyinhibitedskinblotchdiseaseingarlicblub. |
