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Verifif ication the Identity of Garlic from Different Growing Area |
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| รหัสดีโอไอ | |
| Creator | 1. Jirapa Austin 2. Phanphaka Rattanakosol 3. Jantana Chokpachuen 4. Ratchanee Siriyan 5. Suchirat Sakuanrungsirikul 6. Saowanee Ketsakul 7. Auttapon Rukkaphan 8. Tawatchai Nimkingrat |
| Title | Verifif ication the Identity of Garlic from Different Growing Area |
| Publisher | Department of Agriculture |
| Publication Year | 2559 |
| Journal Title | THAI AGRICULTURAL RESEARCH JOURNAL |
| Journal Vol. | 34 |
| Journal No. | 3 |
| Page no. | 253 |
| Keyword | morphological characteristics, chemical compounds, DNA fingerprint, ลักษณะทางสัณฐานวิทยา, องค์ประกอบทางเคมี, ลายพิมพ์ DNA , กระเทียม |
| ISSN | 1258389 |
| Abstract | The aim of this study was to identify variety of garlic from different plantationsarea. The studies were conducted at Si Sa Ket Horticultural Research Center and NanAgricultural Research and Development Center during 2012-2013. Garlics samples werecollected from various planting area to observe the morphological characteristics whichemphasize on the vegetative morphology and storability. Fortyone garlic samples werecollected from commercially cultivated areas. From the results, the storability of garlicbulbs was over 180 days for all samples. Garlic could be identified by visual based onpseudo stem, leaves wide, bulb size and bulb color traits. The samples can be dividedinto two main plantations, from the Northern and the North-eastern area. Thechemical analyses were carried out in eighteen garlic samples. Garlic oils wereextracted using two methods that hot and cold solvent extraction with Ethanol andHexane as solvents determined by GC-MS. The results shown that, different extractionmethod can be found different chemical compositions. GA55018 garlic sample oil wasfound five compounds, Trisulfide, di-2-propenyl was the highest using hot solventextraction. But garlic oil extracted using cold solvent extraction was found more thanten different chemical compounds and Phthalic acid, di-iso-octylester was the highest.It could be suggested that the garlic oil should be extracted using two methods with Hexane as a solvent. From chemical analyses of garlic sample oils were extracted using hot solvent extraction with Hexane as a solvent. It can be separated chemical compounds and itsderivatives into eleven groups based on their chemical structure. Nutrients and minerals of garlic were not different. Si Sa Ket garlics had higher calcium and sodium than garlics from the Northern. DNA fingerprint of garlic using randomly amplified polymorphic DNA (RAPD) andMicrosatellite were studied. As the results, the garlic samples were clustered into two clusters that garlic planted at Si Sa Ket and garlic planted at the Northern. In conclusion, DNAfingerprint is a highly accurate technique for identification of garlic samples. |
