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Restriction Endonuclease Pattern of Thai Bombyx mori L. Nucleopolyhedrovirus |
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| รหัสดีโอไอ | |
| Creator | Mallika Kaewwises |
| Title | Restriction Endonuclease Pattern of Thai Bombyx mori L. Nucleopolyhedrovirus |
| Contributor | Sudawan Chaeychomsri, Srimek chowpongpng, Tipvadee Attathom |
| Publisher | Department of Agriculture |
| Publication Year | 2550 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 25 |
| Journal No. | 1 |
| Page no. | 86-103 |
| Keyword | restriction pattern, nucleopolyhedrovirus, Bombyx mori, silkworm |
| URL Website | https://at.doa.go.th/journal |
| Website title | Thai Agricultural Research Journal |
| ISSN | 0125-8389 |
| Abstract | Bombyx mori nucleopolyhedrovirus (BmNPV) is a causative agent of grasserie which is the most destructive disease of silkworm (Bombyx mori. L.) Restriction profiel of Thai BmNPV destructive disease of silkworm (Bombyx mori. L.) Restriction profile of Thai BmNPV genomic DNA was studied by cleaving the DHA with BamHI, Bg/II, HindIII, Ncol, Pstl, and the minimize of 6, 11, 19, 11 and 17 different DNA fragments wee produced, respectively. Molecular size of the fragments was estimated by summation of the size of DNA fragments generated by restriction exzymes degestion as viewed on the electrophoresed get. The Thai BmNPV genome was estimated to be in the range of 92.3-125.8 kb with the average of 110.6 kb. In comparison with genome of BmNPV T3 and D1 isolates from Japan, and N isolate from India, the Thai BmNPV genome is the smallest in size. |
