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Identification of Durian (Durio spp.) Cultivars, Clones by DNA Amplification Fingerprinting (DAF) |
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| รหัสดีโอไอ | |
| Creator | Songpol Somsri |
| Title | Identification of Durian (Durio spp.) Cultivars, Clones by DNA Amplification Fingerprinting (DAF) |
| Contributor | Suchirat Sakuanrungsirikul, Wanida Ngam-Ngern, Therawut Wongvarat |
| Publisher | Department of Agriculture |
| Publication Year | 2548 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 23 |
| Journal No. | 2 |
| Page no. | 188-210 |
| Keyword | Durio spp., D. zibethinus, identification, DNA fingerprinting, DAF |
| URL Website | https://at.doa.go.th/journal |
| Website title | Thai Agricultural Research Journal |
| ISSN | 0125-8389 |
| Abstract | The polymerase chain reaction (PCR) โ" based DNA amplification fingerprinting (DAF) approach was used to investigate genetic relationships among 9 species of genus Durio, 56 cultivars of common durian (D.zibethinus Murr.) Pairwise similarity matrix were developed using band-sharing data generated by the selected 12 primers out of 180 primers such as Operon A-04, A-06, A-09, A-19, A-20, B-01, B-03, B-16, C-06, G-20, S-01, and S-02. These experiments were conducted at Chanthaburi Horticultural Research Center and Horticulture Research Institute between October 1999- September 2005. In the first experiment with 8 spccies and 18 cultivars, there are 187 polymorphic loci from 217 loci or 86.17% for species and cultivars identification. Second experiment with 9 species and 56 cultivars, 278 polymorphisms were identified from a total of 298 produced bands or 93.29%. The third experiment, 48 polymorphic loci from 192 loci or 25.00% were identified for selected clones of 4 commercial cultivars. Dendrograms were constructed using the unweighted pair group method with arithmetic averages (UPGMA), could separate the genotypes of species and cultivars into 4 and 3 groups, and the genotypes of selected clones into 4 groups for these experiments, respectively. |
