Induction of Embryogenic Callus in Durian via Tissue Culture
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Creator Jaruwan Chartisathian
Title Induction of Embryogenic Callus in Durian via Tissue Culture
Contributor Suphap Sunthronon, Hirum Hirunpradit
Publisher Department of Agriculture
Publication Year 2544
Journal Title Thai Agricultural Research Journal
Journal Vol. 19
Journal No. 1
Page no. 32-43
Keyword tissue culture, somatic embryogenesis, embyogenic callus, durian.
URL Website https://at.doa.go.th/journal
Website title Thai Agricultural Research Journal
ISSN 0125-8389
Abstract Embryogenic callus can be induced from ovule seeds of Durio zibethinus Murr., variety Montong. The appropriate fruit ages wee 8-20 days or 1-3 weeks after anthesis. The older and the younger ones were not induced embryogenic callus. Callus can be obtained from 2 different kinds of induction media, one has been Murashige & Trucker basal medium with 500 mg/.l malt extract, 0.1 mg/I IAA, 1 mg/I IAA, 1 mg./kinetin (Nu 5) and another one has been Murashige & Skoog basal medium with 1 mg./I NAA(MSN). Subculture every 3 weeks can be reduced the phenolic compound productions and obtained to maximum of embryogenic callus proliferation. This technique was also suitable for embryogenic callus in Durio giffithii (Mast.) Bakh., the wild type of durian in Thailand.
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