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Protoplast Isolation and Culture of Aromatic Rice (Oryza sativa L.) Variety KDML 105 |
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| รหัสดีโอไอ | |
| Creator | Prapa Sricpichitt |
| Title | Protoplast Isolation and Culture of Aromatic Rice (Oryza sativa L.) Variety KDML 105 |
| Contributor | Saowaree Tangsakun |
| Publisher | Department of Agriculture |
| Publication Year | 2543 |
| Journal Title | Thai Agricultural Research Journal |
| Journal Vol. | 18 |
| Journal No. | 3 |
| Page no. | 266-279 |
| Keyword | rice (Oryza sativa L.) protoplast isolation, protoplast culture |
| URL Website | https://at.doa.go.th/journal |
| Website title | Thai Agricultural Research Journal |
| ISSN | 0125-8389 |
| Abstract | Protoplasts were isolated from 3 sources of cells; leaves of seedlings, embryo-derived calli and cell suspension by incubating them in enzyme solution containing various kinds and concentrations of enzymes for 1, 3 and 5 hrs, repecitively. The results showed that leaves from 5-day-old seedlings was the most suitable source of cells for protoplast isolation which gave the highest yield of proteplasts 9.14 X 10 per 1 g fresh weight of leaves. The kinds and concentrations of enzymes optimum for protoplast isolation were fifferent depended on the sources of cells used for isolation. the enzyme solution containing 2% cellulase Onozuka RS, 1% macerozyme R 10 and 0.1% pectolyase was suitable for isolation of protoplast from the leaves whereas calli and cell suspension gave high yielded protoplasts when incubated in enzymen souution containing 4% cellulase Onozuka RS, 1% macerozyme R 10 and 0.2% pectolyase. The incubation period of 3 hrs was optimum for protoplast isolation from leaves, calli and cell lsuspension. However, protoplasts isolated from cell suspension possessed higher percentage of viabiligy (80-90%) than protoplasts isolated from leaves and calli (50-60%). The isolated protoplast could not divide to form colony or callus when cultured on N6 or K8P medium |
