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CHENODEOXYCHOLIC ACID INHIBITS CFTR-MEDIATED CHLORIDE SECRETION IN RENAL TUBULAR CELLS |
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| รหัสดีโอไอ | |
| Creator | Sunhapas Soodvilai |
| Title | CHENODEOXYCHOLIC ACID INHIBITS CFTR-MEDIATED CHLORIDE SECRETION IN RENAL TUBULAR CELLS |
| Contributor | Nipitpon Srimai |
| Publisher | Faculty of pharmacy, Silpakorn University |
| Publication Year | 2564 |
| Journal Title | Thai Bulletin of Pharmaceutical Sciences |
| Journal Vol. | 16 |
| Journal No. | 2(July-December)2021 |
| Page no. | พ.ย.-18 |
| Keyword | bile acid, MDCK, chloride channel, chloride secretion, kidney |
| URL Website | https://li01.tci-thaijo.org/index.php/TBPS |
| ISSN | 2586-8659 |
| Abstract | Chenodeoxycholic acid (CDCA), a primary bile acid, has been reported to activate cystic fibrosis transmembrane conductance regulator (CFTR)-mediated Cl- secretion in intestinal cells. However, there have not been any reports on the role of bile acid in the regulation of CFTR-mediated Cl- secretion in renal cells. In this study, we revealed that CDCA reduced CFTR-mediated Cl- secretion in Madin-Darby canine kidney (MDCK) cells, renal tubular cells. Incubation MDCK cell monolayers with 20 ?M CDCA for 48 h led to a decrease in [Arg8]-vasopressin (AVP)-induced Cl-current (ICl-). The inhibition on ICl- was not a result of CDCA-induced toxicity as shown by the treatment of the cells with CDCA for 72 h did not alter cell viability. Interestingly, acute incubation of MDCK cell monolayers with CDCA did not reduce ICl-. Furthermore, basolateral membrane permeabilization of MDCK cell monolayers still decreased apical ICl-, indicating that the target of CDCA might be located at the apical membrane. Western blot analysis revealed that inhibition of Cl- secretion occurred via a decrease in CFTR protein expression. These results reveal that CDCA inhibits Cl- secretion in renal tubular cells via a decrease in CFTR protein expression. |
