AN APPLICATION OF TLC-IMAGE ANALYSIS FOR INVESTIGATION OF AQUEOUS EXTRACT OF COMMON CULINARY HERBAL COMBINATION, KAFFIR LIME LEAF, GALANGAL AND LEMON GRASS RHIZOMES
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Creator 1. Panadda Phattanawasin
2. , Jankana Burana-osot
3. Kanchana Kengkoom
4. Uthai Sotanaphun
Title AN APPLICATION OF TLC-IMAGE ANALYSIS FOR INVESTIGATION OF AQUEOUS EXTRACT OF COMMON CULINARY HERBAL COMBINATION, KAFFIR LIME LEAF, GALANGAL AND LEMON GRASS RHIZOMES
Publisher Faculty of pharmacy, Silpakorn University
Publication Year 2562
Journal Title Thai Bulletin of Pharmaceutical Sciences
Journal Vol. 14
Journal No. 1(January-December)2019
Page no. 27-33
Keyword TLC-image analysis, kaffir lime leaf, galangal rhizome, lemon grass rhizome, fingerprint
URL Website https://www.tci-thaijo.org/index.php/TBPS
ISSN 2586-8659
Abstract Kaffir lime leaf, galangal, and lemon grass rhizomes are ones of the common kitchen herbs used as flavoring ingredients in famous traditional Thai cuisine, called TomYum. These herbs are also employed as herbal medicines for a wide range of applications. A number of studies were conducted to investigate phytochemical constituents of these household remedy, however, the gas chromatographic (GC) and high performance liquid chromatographic (HPLC) analyses of volatile oils or essential oils in each individual herbal extract prepared by low to partial polar organic solvents were mainly focused. Since combinations of these herbs are usually prepared by brewing with hot water, therefore, this study presented the use of TLC-image analysis method for visualization of all detectable constituents in the aqueous extract of the multi-ingredient preparation. Good chromatographic separations of various substance classes were achieved on silica gel 60 F254S TLC plates with the use of several mobile phase compositions, multiple detections and TLC-image analysis software, Sorbfil TLC Videodensitometer, to create a chromatographic profile.
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