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Differentiation of a Hyperthermophilic Archaeon Pyrococcus sp. strain Pikanate 5017, by Arbitrarily Primed PCR |
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| รหัสดีโอไอ | |
| Creator | 1. Patlada Pasomsup 2. Juan Miguel Gonzalez 3. Maria Carmen Portillo 4. Veeranun Pongsapukdee 5. Wirojne Kanoksilapatham |
| Title | Differentiation of a Hyperthermophilic Archaeon Pyrococcus sp. strain Pikanate 5017, by Arbitrarily Primed PCR |
| Publisher | Silpakorn University Research and Development Institute |
| Publication Year | 2554 |
| Journal Title | Silpakorn University Science and Technology Journal |
| Journal Vol. | 5 |
| Journal No. | 1 |
| Page no. | 14-23 |
| Keyword | Thermococcales, Pyrococcus, Thermococcus, fingerprint, hyperthermophile, hot spring |
| ISSN | 1905-9159 |
| Abstract | Pyrococcus and Thermococcus are hyperthermophilic archaea in the order Thermococcales. Both genera are strictly anaerobes, gaining energy by fermentation of peptide and several carbohydrates at optimal temperature above 70 ? C. A Pyrococcus sp. strain Pikanate 5017 (PK 5017) was recently isolated from a hot spring in Northern Thailand. The strain PK 5017 is a valuable source of numerous genes encoding thermostable enzymes. Growth kinetics determined at various temperatures (75-105 ? C) indicates that strain PK5017 is a fast growing archaeon. An arbitrarily primed PCR (AP-PCR) technique was successfully applied to differentiate the genome sequences of six members of Thermococcales. Two single primers, ARB-1f (5' ATGAG GACT GAAA CCATT 3') and ARB-2f (5' GTAAA ACGA CGGC CAGT 3'), are effective in producing polymorphisms of the PCR products at 3-10 ng of DNAs. The unique AP-PCR fingerprints distinguish the strain PK5017 from P. furiosus, P. horikoshii, P. abyssi, T. litoralis, and T. celer. The results indicate a clear distinction of genome sequences among Pyrococcus and Thermococcus genera. |
