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Optimal medium for watercress (Alternanthera sp.) micropropagation |
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| รหัสดีโอไอ | |
| Creator | Vachiraporn Pikulthong |
| Title | Optimal medium for watercress (Alternanthera sp.) micropropagation |
| Contributor | Sirirat Phakpaknam, Manussawee Dechkla,Tharathorn Teerakathiti |
| Publisher | Faculty of Science and Technology, Suan Sunandha Rajabhat University |
| Publication Year | 2561 |
| Journal Title | Suan Sunandha Science and Technology Journal |
| Journal Vol. | 5 |
| Journal No. | 2 |
| Page no. | 29 to 34 |
| Keyword | Alternanthera sp., Micropropagation, Sodium hypochlorite, Benzyladenine |
| URL Website | www.ssstj.sci.ssru.ac.th |
| Website title | Suan Sunandha Science and Technology Journal (SSSTJ) |
| ISSN | 2351-0889 |
| Abstract | The objective of the present study is to find the optimal conditions for micropropagation of Alternanthera sp. The first experiment aims to find the optimal sterilization of lateral bud meristems for in vitro culture. The results revealed that the best sterilization technique is to clean lateral bud meristem by bending shoot and dipping in 70% ethanol for 1 min. These shoots were then removed and immersed in 3% sodium hypochlorite solution for 10 min. This sterilization technique provides the highest survival percentage of 95% and the best microorganism removal. The second experiment aim to determine the effects of the plant growth regulators benzyladenine (BA) on shoot proliferation. The shoot induction of sterile lateral bud meristems of Alternanthera sp. was then carried out in Murashige and Skoog (MS) media supplemented with benzyladenine (BA) at five different concentrations (0, 1, 2, 3 and 4 mg/l) under 25?2 ?C and light condition for 4 weeks. It was found that MS media supplemented with 3 mg/l BA induced the optimal growth and development of shoots. This condition provides the highest root induction and the significant average shoot of 12.88?0.51 shoots/explant (p<0.05). The results from the present study could be the basic information for producing aquatic plants that have economic value and genetic conservation. In additions, it may be useful for biological activity tests or provide the possibility for callus induction for further somatic embryo culture technology. |
