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DNA extraction from Oyster Mushrooms Fruiting Bodies without Mycelial Activation Process |
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| รหัสดีโอไอ | |
| Creator | Rutthaporn Chandet |
| Title | DNA extraction from Oyster Mushrooms Fruiting Bodies without Mycelial Activation Process |
| Contributor | Nirutcha Suriya, Parinyanuch Pinnil, Tipsuda Tangtragoon, Chindanai Aurfurphan |
| Publisher | King Mongkut’s University of Technology Thonburi |
| Publication Year | 2567 |
| Journal Title | Science and Engineering Connect |
| Journal Vol. | 47 |
| Journal No. | 1 |
| Page no. | 3-10 |
| Keyword | Oyster Mushrooms, Molecular Marker, ITS, HAT-RAPD, DNA |
| URL Website | https://journal.kmutt.ac.th/ |
| Website title | เว็บไซต์ Science and Engineering Connect |
| ISSN | 3027-7914 |
| Abstract | Oyster mushrooms (Pleurotus spp.) are economically valuable mushrooms with high market demand. They are popularly consumed worldwide due to their highly palatable taste and numerous medicinal properties. Despite the many benefits of oyster mushrooms, information regarding the genetic diversity of these mushrooms is limited; investigation into their diversity is of utmost importance. While molecular marker techniques, along with the study of phenotypes, are popular approaches, the use of these techniques necessitates the use of high-quality DNA. However, the process of stimulating mycelium growth is complex and time-consuming. A direct DNA extraction method from oyster mushroom fruiting bodies was therefore developed in this present study. The quality of the extracted DNA was evaluated using PCR with ITS and HAT-RAPD molecular markers. The results demonstrated clear and high-quality DNA band patterns, enabling the identification and classification of oyster mushroom species. |
