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Comparative Analysis of ?-Amylase Sequences in Selected AspergillusSpecies and Analysis of Amylase Activity in Three Locally Isolated AspergillusSpecies |
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| รหัสดีโอไอ | |
| Creator | Karen Jing Yi Hon |
| Title | Comparative Analysis of ?-Amylase Sequences in Selected AspergillusSpecies and Analysis of Amylase Activity in Three Locally Isolated AspergillusSpecies |
| Contributor | Nurul Arrisya Aimi Mohd Amran, Afiq Nasri Abdullah, Ngui Sing Ngieng, Hairul Roslan |
| Publisher | Research Administration Division |
| Publication Year | 2567 |
| Journal Title | Science & Technology Asia |
| Journal Vol. | 29 |
| Journal No. | 3 |
| Page no. | 224-243 |
| Keyword | amylase, Amylase activity index, Aspergillus, Phylogenetic tree, Sequence analysis |
| URL Website | https://www.tci-thaijo.org/index.php/SciTechAsia/index |
| Website title | ThaiJo2 |
| ISSN | 2586-9000 |
| Abstract | Amylase is an important enzyme in industries where members of the Aspergillusgenus are significant producers of industrial amylase enzymes;?-amylase is an endoamylase which playsa crucial role in starch hydrolysis. However, knowledge regarding the sequences and evolutionary relationship of ?-amylase among sectionsNigri, Flaviand FumigatiintheAspergillusgenus have remained unknown. The amylase activity of some previously isolated important Aspergillusspecies,namely A. niger, A. fumigatusand A. flavus, also requires further investigation. In this study, ?-amylase sequences of species under three sections intheAspergillusgenus were retrieved from NCBI for sequence analysis and alignment for phylogenetic tree construction. The solid-based method was then applied to screen for amylase production and the Enzymatic Activity Index (EAI)was evaluated to analyze amylase activity. Similarities inenzyme sequences among the selected species were established through the presence of ?-amylase domains and phylogenetic treebranching thatrooted to a common ancestor. This study also found that the amylase activity of three selected species differed significantly, with A. flavushaving the highest EAI of 1.03 ฑ 0.049, followed by A. niger(0.77 ฑ 0.049) and A. fumigatus(0.60 ฑ 0.103). |
