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Efficiency of genetic markers from studies of sea cucumbers (Holothuroidea: Holothuriida) |
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| รหัสดีโอไอ | |
| Creator | Sitthichok Theeratatthanakorn |
| Title | Efficiency of genetic markers from studies of sea cucumbers (Holothuroidea: Holothuriida) |
| Contributor | Narinratana Kongjandtre, Wansuk Senanan, Sumaitt Putchakarn, Thadsin Panithanarak, Sophawadee Muangham, Puttaruk Sananak, Chayanon Chewkit |
| Publisher | Phuket Marine Biological Center |
| Publication Year | 2568 |
| Journal Title | Phuket Marine Biological Center Research Bulletin |
| Journal Vol. | 82 |
| Journal No. | 1 |
| Page no. | 47-54 |
| Keyword | Sea cucumber, Holothuriida, genetic markers, COI, 16S rRNA, 18S rRNA |
| URL Website | https://km.dmcr.go.th/c_266 |
| Website title | PMBC Research Bulletin |
| ISSN | 2697-6323 |
| Abstract | Sea cucumbers are a group of large marine benthic invertebrates, that play an important rolein marine ecosystems. They are also in demand for commercial and pharmaceutical purposes. The mosttraded sea cucumbers are those in the order Holothuriida, making them a particularly interesting subjectfor study. Initially, morphological methods were used to study these sea cucumbers, and then geneticinformation has since been incorporated into the research. The aim of this study is to investigate the geneticdifferentiation and evaluate the effectiveness of genetic markers in contributing to the taxonomy of seacucumbers within the order Holothuriida. Nucleotide sequence data was collected from the National Centerfor Biotechnology Information (NCBI), and the sequences were edited and aligned using BioEdit 7.2 andMEGA 11 software. The results indicate that the cytochrome c oxidase subunit I (COI) genetic marker issuitable for species identification but does not perform well in resolving phylogenetic relationships. Incontrast, the 16S rRNA genetic marker can be used for both species identification and for examiningrelationships between different species within the genera. The 18S rRNA genetic marker is not suitablefor species identification but can be used to study evolutionary relationships in order or family levels.Therefore, the choice of genetic marker depends on the specific objectives of the research. |
