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Proteomics analysis of rat testis reveals changes of proteins involving the signal transduction after methamphetamine exposure |
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| รหัสดีโอไอ | |
| Creator | Samur Thanoi |
| Title | Proteomics analysis of rat testis reveals changes of proteins involving the signal transduction after methamphetamine exposure |
| Contributor | Jitnapar Thongleart1, PaweenaKaewman, Sittiruk Roytrakul, Sutisa Nudmamud-Thanoi |
| Publisher | University of Phayao |
| Publication Year | 2564 |
| Journal Title | Naresuan Phayao Journal |
| Journal Vol. | 14 |
| Journal No. | 3 |
| Page no. | 14-25 |
| Keyword | Methamphetamine, Proteomics, Signal transduction, Testis, Rat |
| ISSN | 1906-2141 (Print);2697-4401 (Online) |
| Abstract | Methamphetamine (METH) is an addictive drug potentially affecting the male reproductive system. It causes poor sperm quality and an increase in apoptotic cells within the seminiferous tubule. METH administration can also result in the changes of dopamine, norepinephrine, and GABA in rat testis. These findings provide the hypothesis that METH might associate with the changes of proteins involving biological process in spermatogenesis. Therefore, the aim of this study is to investigate the expression of the signal transduction proteins underlying biological processes in the testis of METH-administered rats using proteomics analysis. Male Sprague-Dawley rats in a control group were received normal saline for 15 days, whereas those rats in an ED-binge METH group were received an escalating dose of METH for 14 days following a binge dose of METH on day 15. Proteins were extracted from the rat testis, which were pooled from three samples in each group. The liquid chromatography-tandem mass spectrometry was performed to identify the protein profiles. The total of 383 proteins were identified in the testis of both groups. 38 proteins were mapped in the signal transduction sub-class underlying biological processes including 19 proteins with up-regulated expression and 19 proteins with down-regulated expression. Moreover, there were 8 proteins responding to METH exposure. In conclusion, our findings will be useful for better understanding of METH on protein expression resulting in the impairment of spermatogenesis in the testis. |
