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Study on hepatoprotective effect of Moringa oleifera Lam. leaves to prevent hepatocyte lesion induced by CCl4 in the HepG2 cell line |
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| รหัสดีโอไอ | |
| Creator | 1. L.X. Le 2. L.K.T. Huynh 3. T.H.T. Do |
| Title | Study on hepatoprotective effect of Moringa oleifera Lam. leaves to prevent hepatocyte lesion induced by CCl4 in the HepG2 cell line |
| Publisher | Faculty of Pharmacy, Mahidol University |
| Publication Year | 2559 |
| Journal Title | Mahidol University Journal of Pharmaceutical Sciences |
| Journal Vol. | 43 |
| Journal No. | 3 |
| Page no. | 153-163 |
| Keyword | HepG2,in vitro,hepatoprotective,CCl4, Moringa oleifera Lam. leaves |
| ISSN | 0125-1570 |
| Abstract | Moringa oleifera Lam. is rich of strong antioxidant reagents, especially isoquercitrin. The present study was an attempt to prove hepatoprotective ability of isoquercitrin (IQ), ethyl acetate (EM) and 70% alcoholic extracts (AM) from M. oleifera leaves against CCl4 induced hepatotoxicity in HepG2 cells. Cells were treated with 3 different samples, including IQ, EM or AM at concentrations corresponding to that of isoquercitrin at 2.5, 5, 10 ?g/ml (C1, C2 and C3, respectively) alone or combined with 2 mM CCl4 for 24h. Hepatoprotective effect was evaluated through assays including cell proliferation, lipid accumulation, necrosis, oxidative stress and apoptosis activation. Results showed that CCl4 reduced 23.2% of HepG2 cells viability. Anti-proliferative effect was prevented in presence of IQ, EM, AM at 3 tested concentrations. |
