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High performance liquid chromatography method for implementation of therapeutic drug monitoring of Vancomycin at Hospital for Tropical Diseases - Ho Chi Minh City Vietnam |
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| รหัสดีโอไอ | |
| Creator | 1. PV. Toi 2. NDK. Tho 3. PN. Phuong 4. HP. Thao 5. TT. Quynh 6. HTM. Thuy 7. DTH. Hanh 8. NVV. Chau 9. GE. Thwaites 10. TT. Hien |
| Title | High performance liquid chromatography method for implementation of therapeutic drug monitoring of Vancomycin at Hospital for Tropical Diseases - Ho Chi Minh City Vietnam |
| Publisher | Faculty of Pharmacy, Mahidol University |
| Publication Year | 2558 |
| Journal Title | Mahidol University Journal of Pharmaceutical Sciences |
| Journal Vol. | 42 |
| Journal No. | 4 |
| Page no. | 186-194 |
| Keyword | Vancomycin,Therapeutic Drug Monitoring (TDM),HPLC,SPE,FPIA |
| Abstract | The simple, rapid and reliable method of high performance liquid chromatography with diode array detection (DAD) for the quantification of vancomycin in human plasma using trimethoprim as an internal standard has been developed and validated. The drug and IS were extracted by solid phase extraction with ISOLUTE-cartridges C8, IST (50mg/mL) and analyzed on Poroshell 120, C8 EC, 2.7?m, 100mm?4.6mm i.d using a mobile phase of potassium dihydrogen phosphate buffer 25mM, pH 3.0 ? acetonitrile (92:8, v/v) at a flow rate of 1mL/min at 40oC. The injection volume was 20?L and the DAD wavelength was set at 236nm. The method was validated over the concentration range of 0.5-100 ?g/mL (r2>0.999) with a limit of detection of 0.2?g/mL and limit of quantification of 0.5?g/mL. Intra-assay and inter-assay precision for VAN at three concentrations (low/medium/high) ranged from 1.89 to 6.5% (%CV), and accuracy varied from 99.1 to 103.4%. VAN and TMP (IS) had high recovery (98.5% and 94% respectively). |
