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Inhibition mechanism of podocyte apoptosis in rats with membranous nephropathy by Chinese medicine Ke-mo-fang through integrin-linked kinase signalling pathway |
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| รหัสดีโอไอ | |
| Creator | Shoulin Zhang |
| Title | Inhibition mechanism of podocyte apoptosis in rats with membranous nephropathy by Chinese medicine Ke-mo-fang through integrin-linked kinase signalling pathway |
| Contributor | Mingyu He, Shuai Zhang, Lindi Zhang, He Nan, Di Jin |
| Publisher | Maejo University |
| Publication Year | 2567 |
| Journal Title | Maejo International Journal of Science and Technology |
| Journal Vol. | 18 |
| Journal No. | 3 |
| Page no. | 252 |
| Keyword | Ke-mo-fang, podocyte apoptosis, membranous nephropathy, integrin-linked kinase |
| Website title | Maejo International Journal of Science and Technology |
| ISSN | 1905-7873 |
| Abstract | Membranous nephropathy (MN), a common pathological type of adult nephrotic syndrome, causes up to 30% of patients to progress to end-stage renal disease within 5-10 years. Podocyte injury and proteinuria are closely related to the occurrence and development of MN, and podocyte epithelial-mesenchymal transition (EMT) is the key factor leading to podocyte injury and proteinuria. Integrin-linked kinase (ILK) is a serine/threonine protein kinase with diverse biological activities. As an important downstream effector molecule of transforming growth factors 1 signalling pathway, ILK induces the specific expression of fibroblasts, accelerates the formation of renal interstitial fibrosis, and plays a role in the EMT of renal epithelial cells. Ke-mo-fang (KMF) is a traditional Chinese medicine formula, which has been used to delay the progression of worsening renal function. However, the therapeutic mechanism of KMF in MN remains unclear. In this study cationic bovine serum albumin was injected at multiple points subcutaneously in axilla, groin and tail vein to establish the MN rat mode. Thereafter, the KMF and ILK inhibitors (OSU-T315) were administered. Through the detection of serum indexes content, the expression of proteins related to kidney pathology, the podocyte function and the ILK signalling pathology, it was confirmed that KMF has a protective effect on podocytes of rats with MN by regulating the ILK signalling pathway, effectively preventing the progression of MN. |
