|
Molecular mechanisms of resveratrol-induced apoptosis in human pancreatic cancer cells |
|---|---|
| รหัสดีโอไอ | |
| Creator | 1. Napaporn Kaewdoungdee 2. Chariya Hahnvajanawong 3. Benjamart Chitsomboon 4. Wongwarut Boonyanugomol 5. Banchob Sripa 6. Kovit Pattanapanyasat 7. Anirban Maitra |
| Title | Molecular mechanisms of resveratrol-induced apoptosis in human pancreatic cancer cells |
| Publisher | Maejo University |
| Publication Year | 2557 |
| Journal Title | Maejo International Journal of Science and Technology |
| Journal Vol. | 8 |
| Journal No. | 3 |
| Page no. | 251 |
| Keyword | pancreatic cancer (Panc 2.03) cell,resveratrol,apoptosis,S phase arrest,Bcl-2,Bax,surviving,apoptosis-inducing factor |
| ISSN | 1905-7873 |
| Abstract | Resveratrol is a polyphenolic phytoalexin found at high concentrations in grapes, nuts, fruits and red wine with reported anti-carcinogenic effects. In this study, the molecular mechanism of resveratrol-induced apoptosis in human pancreatic cancer (Panc 2.03) cells is investigated. Resveratrol treatment of Panc 2.03 cells results in dose-dependent inhibition of cell growth and cells accumulated at the S phase transition of the cell cycle. The anti-proliferative effect of resveratrol is due to apoptosis as seen by the appearance of chromatin condensation, nuclear fragmentation, DNA ladder formation and increased annexin V-stained cells. The apoptotic process is induced by decreased Bcl-2 expression concomitant with increased Bax expression, leading to an increase in the Bax/Bcl-2 ratio and subsequent activation of caspase-9 and caspase-3. In addition, resveratrol treatment also decreases the survivin level and increases the apoptosis-inducing factor level in a dose-dependent manner. These results suggest that resveratrol induces apoptosis of Panc 2.03 cells, at least in part through a mitochondrial-associated intrinsic pathway in both caspasedependent and independent manners. The present findings suggest that resveratrol has potential as a chemopreventive agent, and possibly as a therapeutic one against pancreatic cancer. |
