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Simultaneous determination of plasma lopinavir and ritonavir by chemometrics-assisted spectrophotometry and comparison with HPLC method |
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| รหัสดีโอไอ | |
| Creator | 1. Chutima Matayatsuk Phechkrajang 2. Salinthip Jarusintanakorn 3. Kittisak Sripha 4. Prapin Wilairat |
| Title | Simultaneous determination of plasma lopinavir and ritonavir by chemometrics-assisted spectrophotometry and comparison with HPLC method |
| Publisher | Maejo University |
| Publication Year | 2556 |
| Journal Title | Maejo International Journal of Science and Technology |
| Journal Vol. | 7 |
| Journal No. | 2 |
| Page no. | 248 |
| Keyword | protease inhibitor,lopinavir,ritonavir,chemometrics,PLS-1 |
| ISSN | 1905-7873 |
| Abstract | Chemometrics-assisted spectrophotometry for the determination of two protease inhibitors, lopinavir and ritonavir, in plasma was evaluated. A set of calibration mixtures (calibration set) was designed according to central composite design. The UV spectra obtained from the calibration set were subjected to partial least square regression (PLS-1) to construct the prediction models for lopinavir and ritonavir in unknown samples, which were then validated in a randomly selected set of synthetic mixtures of the drugs. An optimum model was obtained in the wavelength ranges of 215249 nm and 240279 nm with principal components 8 for both lopinavir and ritonavir respectively. The prediction models were used to analyse the two drugs in plasma and the results were compared with those obtained by high-performance liquid chromatography (HPLC). The PLS-1 model and the HPLC method were found to be comparable. |
