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CHARACTERIZATION OF MANNANASE FROM POTENTIAL MANNAN-DEGRADING BACTERIA |
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| รหัสดีโอไอ | |
| Creator | Kwankanit Intaratrakul |
| Title | CHARACTERIZATION OF MANNANASE FROM POTENTIAL MANNAN-DEGRADING BACTERIA |
| Publisher | Pibulsongkram Rajabhat University |
| Publication Year | 2568 |
| Journal Title | Life Sciences and Environment Journal |
| Journal Vol. | 26 |
| Journal No. | 1 |
| Page no. | 51-62 |
| Keyword | enzyme characterization, mannan, mannanase, mannanase-producing bacteria |
| URL Website | https://ph01.tci-thaijo.org/index.php/psru/index |
| Website title | Life Sciences and Environment Journal |
| ISSN | 2773-9201 |
| Abstract | Mannanase is an enzyme that catalyzes the hydrolysis of mannan polysaccha-rides and has applications in diverse industries. It can be produced by various micro-organisms, particularly bacteria, with each strain exhibiting unique enzymatic proper-ties. This study aimed to select a potential mannanase-producing bacterial strain from isolates obtained from coconut residue-rich sites. In addition, identification of bacterial strain and enzyme characterization were performed. The results showed that isolate KD109 exhibited the highest enzyme activity at 1.58 U/ml. Based on 16S rRNA gene sequencing, KD109 showed over 99% sequence identity with Klebsiella pneumoniae. Characterization of the mannanase produced by this strain revealed that the optimum temperature of 50 °C and the optimum pH of 6. Substrate specific-ity analysis showed that the enzyme exhibited the highest specificity for mannan-based substrates, including konjac, followed by locust bean gum. Furthermore, this enzyme exhibited the activity on copra meal, a mannan rich by-product. This study indicated that K. pneumoniae KD109 could be a promising source of mannanase with potential applications in the degradation of mannan-containing raw materials for various industrial processes. |
