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EFFECT OF THE CRUDE PIGMENT EXTRACT FROM ACTINOBACTERIA ISOLATED FROM STINGLESS BEE, TETRAGONILLA COLLINA (SMITH, 1857), ENTRANCE NEST ON NATURAL DYEING FIBERS |
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| รหัสดีโอไอ | |
| Creator | Naruemol Thurnkul1 |
| Title | EFFECT OF THE CRUDE PIGMENT EXTRACT FROM ACTINOBACTERIA ISOLATED FROM STINGLESS BEE, TETRAGONILLA COLLINA (SMITH, 1857), ENTRANCE NEST ON NATURAL DYEING FIBERS |
| Contributor | Sittichai Urtgam, Touchkanin Jongjitvimol, Tawatchai Sumpradit |
| Publisher | Pibulsongkram Rajabhat University |
| Publication Year | 2566 |
| Journal Title | Life Sciences and Environment Journal |
| Journal Vol. | 24 |
| Journal No. | 1 |
| Page no. | 183-195 |
| Keyword | Actinobacterial pigment, Stingless bee entrance nest, Dyeing, Natural fibers |
| URL Website | https://ph01.tci-thaijo.org/index.php/psru/index |
| Website title | Life Sciences and Environment Journal |
| ISSN | 2773-9201 |
| Abstract | Actinobacterial dyes are alternatives for natural fiber dyeing. In this study, the strain C4 isolated from stingless bee entrance nest was evaluated for use as a dye in the textile dyeing process. Morphologically, the strain C4 colonies grown on sodium caseinate agar (SCA) produced water-soluble violet-colored pigment. To identify the actinobacterial strain C4, phylogenetic identification was based on the partial 16S rDNA sequence analysis. The result showed that the strain was identical to Streptomyces californicus (NR 115432) (100% sequence identity). To scale up actinobacterial dye production, strain C4 was cultivated on media supplemented with broken-milled rice as the carbon and energy source. Following actinobacterial growth, the pigment was extracted by 70 % (v/v) ethanol to obtain the actinobacterial dye. The 5 natural fibers, silk, wool, cotton fiber, hemp fiber, and the mixed banana and cotton fiber, were soaked in the actinobacterial dyes for 12 hr after which color measurement was done, following the CIELAB system and the Standard Color Chart R.H.S for natural fibers. The dyeing results indicated that silk showed the highest CIELAB value indicating that it is the most suitable natural fiber for actinobacterial dyeing. It is suggested that the use of actinobacterial dye in the textile industry should be evaluated in future. |
