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PRODUCTION AND ANTIOXIDANT ACTIVITY OF PROTEIN HYDROLYSATE FROM RICE SEED KERNEL |
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| รหัสดีโอไอ | |
| Creator | Chanthakan Nuchsuk |
| Title | PRODUCTION AND ANTIOXIDANT ACTIVITY OF PROTEIN HYDROLYSATE FROM RICE SEED KERNEL |
| Contributor | Supawadee Kaewphokha |
| Publisher | Pibulsongkram Rajabhat University |
| Publication Year | 2564 |
| Journal Title | Life Sciences and Environment Journal |
| Journal Vol. | 22 |
| Journal No. | 2 |
| Page no. | 320-331 |
| Keyword | Rice seed kernel, Protein hydrolysate, Antioxidant activity |
| URL Website | https://ph01.tci-thaijo.org/index.php/psru/index |
| Website title | Life Sciences and Environment Journal |
| ISSN | 2773-9201 |
| Abstract | The purposes of this research were to produce and study the antioxidant activity of protein hydrolysate from seed kernel of four rice cultivars: Ayutthaya 1, Suphanburi 1, Pathum Thani 1 and RD 29. To obtain crude protein extract, rice seed kernel was soaked in 0.2 % sodium hydroxide (NaOH) and protein content was determined according to the Lowry method. The results showed that crude protein extract from seed kernel of Pathum Thani 1 had the highest percent yield (8.01 ± 0.08 %) and the highest amount of crude protein (6.92 ± 0.02 g/100 g sample). The protein types and patterns were analyzed by SDS-PAGE technique. Electrophoretic patterns of the extracted proteins have shown a slight difference in the patterns of proteins with molecular weight between 19.3 – 102.1 kDa. The production of protein hydrolysate was prepared by hydrolysis of crude protein extract with 4 M HCl at 100C for 6 h and antioxidant activity was analysis by DPPH method. Th results showed protein hydrolysate had higher scavenging activity than crude protein extract for all cultivars. |
