|
Immunological and molecular detection of Brucella abortus in buffaloes (Bubalus bubalis) |
|---|---|
| รหัสดีโอไอ | |
| Creator | 1. Kanta Rani 2. Anju Manuja 3. Renu Singh 4. Pramil Kumar |
| Title | Immunological and molecular detection of Brucella abortus in buffaloes (Bubalus bubalis) |
| Publisher | International Buffalo Information Centre, Office of The University Library, Kasetsart University, Bangkok (Thailand) |
| Publication Year | 2557 |
| Journal Title | Buffalo Bulletin |
| Journal Vol. | 33 |
| Journal No. | 1 |
| Page no. | 25-31 |
| Keyword | Buffaloes,Bubalus bubalis,Brucella abortus,Immunological detection,Molecular detection,Dot-ELISA,PCR,RBPT,India |
| ISSN | 1256726 |
| Abstract | Brucellosis is a zoonotic disease of livestock species, wild animals and human beings which causes birth of unthrifty newborn and orchitisepididymitis with frequent sterility in affected animals and undulant fever in human beings. In the present study a total of 73 serum samples were taken from buffaloes and bulls from organized as well as rural areas, eighteen of which had tested previously as positive samples by AB-ELISA. Out of these eighteen, four (22.22 percent) were found to be positive using dot-ELISA, while none of these were found to be positive with RBPT. For rest of the 55 samples both assays (RBPT and dot-ELISA) gave the same results: 12.72 percent of the samples were found to be positive however, positivity was higher in case of dot-ELISA. In three (5.45 percent) out of seven positive samples Brucella specifi c DNA was detected in blood using PCR while in milk samples, no positive results were observed. The same results were observed through serological testing. |
