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Genetic diversity and conservation of animal genetic resources in Iraqi buffalo using microsatellite markers |
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| รหัสดีโอไอ | |
| Creator | 1. Talib Ahmed Jaayid 2. Maytham Abdul Kadhim Dragh |
| Title | Genetic diversity and conservation of animal genetic resources in Iraqi buffalo using microsatellite markers |
| Publisher | International Buffalo Information Centre, Office of The University Library, Kasetsart University, Bangkok (Thailand) |
| Publication Year | 2557 |
| Journal Title | Buffalo Bulletin |
| Journal Vol. | 33 |
| Journal No. | 3 |
| Page no. | 271-276 |
| Keyword | Iraqi buffalo,Bubalus bubalis,Microsatellites,Genetic diversity,PCR,Genetic variability,Heterozygosity,Blood,Iraq |
| ISSN | 1256726 |
| Abstract | In our study, conducted in Iraq and Huazhong University, China, divided Iraq into three main regions: a southern area including Basrah, Missan, and Dhi-Qar, a middle area including Al-Qadisiyah, Babil, Karbala and Baghdad, and a northern area including Diyala, Kirkuk and Mosul. The aim of the study was to measure the genetic diversity, polymorphism and heterozygosity in Iraqi buffaloes using microsatellite techniques. Sixty-nine blood samples were collected from unrelated animals. Six microsatellite markers were used (ETH125, CSSM060, BM1706, ETH02, ETH225 and INRA005). The polymerase chain reaction (PCR) was done using specific bovine primers and a genetic analyzer (ABI-3730) Our results revealed that all the six markers amplified the DNA. The marker INRA005 did not show high polymorphism, it only revealed three alleles (137-141 bp). The marker ETH152 showed the highest level of polymorphism, it has sixteen alleles ranged between 192-217 bp. This study showed that there are three main clusters: the first one included Basrah, Baghdad and Al-Qadisiyah, the second consisted of Kirkuk and Missan, while the third consisted of Babil and Mosul. |
