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Effect of pre-partum supplementation of vitamin E to Murrah buffaloes on lymphocyte function of calves |
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| รหัสดีโอไอ | |
| Creator | 1. Singh, A.K. 2. Pandita, S. 3. Upadhyay, R.C. 4. Chandra, G. |
| Title | Effect of pre-partum supplementation of vitamin E to Murrah buffaloes on lymphocyte function of calves |
| Publisher | International Buffalo Information Centre, Office of The University Library, Kasetsart University, Bangkok (Thailand) |
| Publication Year | 2557 |
| Journal Title | Buffalo Bulletin |
| Journal Vol. | 33 |
| Journal No. | 2 |
| Page no. | 233-243 |
| Keyword | Murrah buffaloes,Buffalo calf,Bubalus bubalis,Cell mediated immunity,Lymphocytes,Nitric oxide,Supplementation,Vitamin E,India |
| ISSN | 1256726 |
| Abstract | The present study was undertaken in buffalo neonates born to vitamin E supplemented (treatment group) and non-supplemented (control group) Murrah buffaloes. Blood samples were collected into sterile vacutainer tubes from calves at day 0 (before colostrum feeding), 7, 28, and 126-post birth. The lymphocytes were separated and cultured in RPMI 1640 medium with mitogen phytohemagglutinin-P (PHA-P) for 36h at 37 deg C in a humidified CO2 incubator (95 percent air and 5 percent CO2). The lymphocyte responsiveness in vitro evaluated in response to different nitric oxide compounds viz. S-Nitroso-N-acetylpenicillamine (SNAP), N omega-Nitro-L-arginine methyl ester (L-NAME) and L-NG-Monomethyl-arginine (NMMA). The blastogenic response between days in both groups varied significantly (P LT 0.01) but it was not found to be significant between different groups. The precolostrum stimulation index was significantly low (P LT 0.05) in all the calves. The mitogen induced lymphocyte blastogenesis was not affected by low levels (1.0 mM) of SNAP but significantly inhibited (P LT 0.001) at 10 mM in both the groups. At 10 mM of L-NAME the blastogenic response between days in both groups varied signifi cantly (P LT 0.01) but it was not found to be significant at 10 mM of L-NMMA. Nitric oxide (NO) production by lymphocytes from all calves increased (P LT 0.01) progressively with age. The lymphocytes supplemented with SNAP (1.0 and 10 mM) significantly (P LT 0.001) produce higher amounts of NO in comparison to non-supplemented lymphocytes. At a low level (0.2 mM) of L-NAME and L-NMMA, the change in the NO production between days was significant in both the groups. However, at a higher level (10 mM) of L-NAME and L-NMMA, the differences were not found to be signifi cant between days and between the two groups. The study demonstrates that buffalo calves lymphocytes have an active nitric oxide synthase (NOS) system. The present study also indicates that the immune competence of buffalo calf lymphocytes is not affected by prepartum supplementation of vitamin E to buffaloes. |
