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Prevalence of coagulase gene positive Staphylococcus aureus bovine mastitis in three distinct geoclimatic regions of India |
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| รหัสดีโอไอ | |
| Creator | 1. Sahu, B.D. 2. Mukherjee, R. 3. Ajay Kumar 4. Amit Kumar 5. Jyoti Sahu |
| Title | Prevalence of coagulase gene positive Staphylococcus aureus bovine mastitis in three distinct geoclimatic regions of India |
| Publisher | International Buffalo Information Centre, Office of The University Library, Kasetsart University, Bangkok (Thailand) |
| Publication Year | 2557 |
| Journal Title | Buffalo Bulletin |
| Journal Vol. | 33 |
| Journal No. | 2 |
| Page no. | 208-214 |
| Keyword | Buffaloes,Bubalus bubalis,Cattles,Bos indicus,Bovine mastitis,Coagulase gene,Geoclimatic zones,Staphylococcus aureus,Polymerase chain reaction,India |
| ISSN | 1256726 |
| Abstract | A two-year retrospective study was performed to determine the bacteriological aspects of coagulase positive Staphylococcal mastitis by bacterial culture and by polymerase chain reaction using specifi c primers in cattle and buffalo farms from three distinct geoclimatic zones of India. A total of 3934 quarters from 1022 cattle and buffaloes were screened from the Bareilly, Kumaoun and Rajnanadgoan regions of India. Mastitis cases were detected from 754 quarter milk samples on the basis of CMT positive, SCC GT 7*10**(5) cells /ml and milk positive on bacterial culture. The overall prevalence of Staphylococcal mastitis (BSM) was 22.02 percent on cow basis and 8.64 percent on quarter basis in 1022 lactating cows with 3934 functional quarters. The intra-mammary infection (IMI) was higher to the extent of 45.54 percent in the Bareilly and 56.31 percent in the Rajnandgoan regions as compared to the Mukteshwar region. The coagulase gene of S. aureus was amplified with a pair of primers using the polymerase chain reaction (PCR). PCR revealed the cao gene from 14.73 percent, 10.53 percent and 13.24 percent more mastitic milk samples as compared to the bacterial culture method. Amplifi cation of the coa gene by PCR is an important technique for quick diagnosis of prevalent bacterial pathogens of a particular region. With the help of PCR, a large number of lactating dairy animals can be screened with accuracy in less time in the study area for implementing therapeutic and preventive measures. |
