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Cloning and sequencing of gC gene of bovine herpesvirus 1 (BHV1) from buffalo bull semen |
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| รหัสดีโอไอ | |
| Creator | 1. Supriya Pal 2. Mayur K. Jhala 3. Pranay Kumar 4. Chaitanya G. Joshi |
| Title | Cloning and sequencing of gC gene of bovine herpesvirus 1 (BHV1) from buffalo bull semen |
| Publisher | International Buffalo Information Centre, Office of The University Library, Kasetsart University, Bangkok (Thailand) |
| Publication Year | 2557 |
| Journal Title | Buffalo Bulletin |
| Journal Vol. | 33 |
| Journal No. | 2 |
| Page no. | 164-169 |
| Keyword | Buffalo bull,Bubalus bubalis,Cloning,Semen,Bovine herpesvirus 1,BHV1,gC gene |
| ISSN | 1256726 |
| Abstract | The present study was aimed to characterize the local field bovine herpesvirus 1 (BHV1) by cloning and sequencing of the glycoprotein C (gC) gene segment of BHV1, amplified by the polymerase chain reaction (PCR). The PCR product of 173 bp of a representative positive semen sample was cloned using the pTZ57R/T vector supplied with the InsT/Aclone TM PCR product cloning kit. The cloned white colonies were confirmed by M13 PCR, which yielded an expected amplified product of 327 bp. The cloned PCR product of the field BHV1 gC gene was sequenced and then analysed by in silico analysis. The consensus sequence of 168 bp obtained was compared with the six published GenBank sequences, and it was found that the field BHV1 from Gujarat showed a range of homology (89 percent to 92 percent) with maximum identity (92 percent) with the BHV1 of three European and one North American isolates. The sequence obtained showed deletions at two positions i.e. 40 and 70 nucleotides and point mutations at seven different positions (2, 61, 116, 134, 143, 151 and 155) as compared to the six published sequences. Such alterations in the sequence indicated frameshift mutations in the gC gene region, with the corresponding alteration in amino acid (aa) sequence. |
