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Study of leptin gene polymorphism in Mehsana buffaloes (Bubalus bubalis) |
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| รหัสดีโอไอ | |
| Creator | 1. Manisha Deshpande 2. Rank, D.N. 3. Vataliya, P.H. 4. Joshi, C.G. |
| Title | Study of leptin gene polymorphism in Mehsana buffaloes (Bubalus bubalis) |
| Publisher | International Buffalo Information Centre, Office of The University Library, Kasetsart University, Bangkok (Thailand) |
| Publication Year | 2557 |
| Journal Title | Buffalo Bulletin |
| Journal Vol. | 33 |
| Journal No. | 1 |
| Page no. | 115-119 |
| Keyword | Mehsana buffaloes,Bubalus bubalis,PCR-RFLP,OLR1 gene,Leptin gene,Polymorphism,Molecular genotyping,Cloning,India |
| ISSN | 1256726 |
| Abstract | Leptin is a hormone which is mainly produced in adipose tissue and secreted in to blood stream as a 16 kDa protein. It plays a key role in the regulation of food intake, energy expenditure, fertility and immune functions. Leptin binds to a receptor mainly localized on neuropeptide Yneurons, which results in reduction of feed intake and increase of energy expenditure. Neuropeptide Y is also involved in the control of reproductive functions. This study was aimed to reveal PCRRFLP pattern of the leptin (exon 2) locus in the Mehsana breed of buffaloes. Fragments of leptin 331 bp and OLR1-288bp were amplified by PCR, and subsequently RFLP study was carried out to identify genotypes of the animals with HphI restriction enzyme. Further representative samples were cloned and in vector, and after sequencing, sequence variation in nucleotide sequences of leptin was analysed. |
